ELISA Kit for Nuclear Factor Kappa B (NFkB)

0.312-20ng/mL

The minimum detectable dose of this kit is typically less than 0.114ng/mL

3h

This assay has high sensitivity and excellent specificity for detection of Nuclear Factor Kappa B (NFkB).
No significant cross-reactivity or interference between Nuclear Factor Kappa B (NFkB) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor Kappa B (NFkB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Factor Kappa B (NFkB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Factor Kappa B (NFkB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nuclear Factor Kappa B (NFkB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Proanthocyanidins from grape seeds modulates the nuclear factor-kappa B signal transduction pathways in rats with TNBS-induced recurrent ulcerative colitis; New Method to Isolate and Culture Rat Kupffer Cells; Long-term aspirin pretreatment in the prevention of cerulein-induced acute pancreatitis in rats; Cilostazol Renoprotective Effect: Modulation of PPAR-γ, NGAL, KIM-1 and IL-18 Underlies Its Novel Effect in a Model of Ischemia-Reperfusion; Preventive use of Lactobacillus plantarum LS/07 and inulin to relieve symptoms of acute colitis.; Use Of The Prebiotic Inulin In The Prevention Of Adverse Signs Of Acute Colitis; Protective Effect of Oral Hesperetin Against Unilateral Striatal 6-Hydroxydopamine Damage in the Rat; THE PROPHYLACTIC EFFICIENCY OF LYCOPENE AGAINST GAMMA IRRADAITION-INDIUCED CARDIAC OXIDATIVE DAMAGE, PATHOLOGY AND APOPTOSIS IN RATS; Carnosine ameliorates cognitive deficits in streptozotocin-induced diabetic rats: Possible involved mechanisms.; Forskolin, a hedgehog signalling inhibitor, attenuates carbon tetrachloride-induced liver fibrosis in rats.; Garlic active constituent s-allyl cysteine protects against lipopolysaccharide-induced cognitive deficits in the rat: Possible involved mechanisms.; S-allyl cysteine ameliorates cognitive deficits in streptozotocin-diabetic rats via suppression of oxidative stress, inflammation, and acetylcholinesterase.; Ellagic acid ameliorates learning and memory deficits in a rat model of Alzheimer's disease: an exploration of underlying mechanisms.; Acetyl-l-carnitine protects dopaminergic nigrostriatal pathway in 6-hydroxydopamine-induced model of Parkinson's disease in the rat.; Diosgenin ameliorates development of neuropathic pain in diabetic rats: Involvement of oxidative stress and inflammation.; Sildenafil protects against bile duct ligation induced hepaticfibrosis in rats: Potential role for silent information regulator 1 (SIRT1); Immunomodulatory Activities of a Fungal Protein Extracted from Hericium erinaceus through Regulating the Gut Microbiota; The shear wave elastic modulus and the increased nuclear factor kappa B(NF-kB/p65) and cyclooxygenase-2 (COX-2) expression in the area of myofascial trigger points activated in a rat model by blunt trauma to the vastus medialis; In vivo cellular and molecular gastroprotective mechanisms of chrysin; Emphasis on oxidative stress, inflammation and angiogenesis; Docking Studies and Biological Evaluation of a Potential β-Secretase Inhibitor of 3-Hydroxyhericenone F from Hericium erinaceus.; Berberine ameliorates intrahippocampal kainate-induced status epilepticus and consequent epileptogenic process in the rat: Underlying mechanisms; Scutellarin alleviates lipopolysaccharide-induced cognitive deficits in the rat: Insights into underlying mechanisms.; Naringenin ameliorates learning and memory impairment following systemic lipopolysaccharide challenge in the rat; Anti-inflammatory potential of Lactobacillus plantarum LS/07 in acute colitis in rats; The potential antifibrotic impact of apocynin and alpha-lipoic acid in concanavalin A-induced liver fibrosis in rats: Role of NADPH oxidases 1 and 4; Effect of Dachaihu Decoction on non-alcoholic fatty liver disease model rats induced by a high-fat high-sugar diet; Amitriptyline attenuates bleomycin-induced pulmonary fibrosis: modulation of the expression of NF-κβ, iNOS, and Nrf2; Complex interaction of dietary fat and Alaskan bog blueberry supplementation influences manganese mediated neurotoxicity and behavioral impairments; Increased cerebral nuclear factor kappa B in a complex regional pain syndrome rat model: possible relationship between peripheral injury and the brain;