ELISA Kit for Mucin 5 Subtype AC (MUC5AC)

78-5, 000pg/mL

The minimum detectable dose of this kit is typically less than 30pg/mL

3h

This assay has high sensitivity and excellent specificity for detection of Mucin 5 Subtype AC (MUC5AC).
No significant cross-reactivity or interference between Mucin 5 Subtype AC (MUC5AC) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 5 Subtype AC (MUC5AC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucin 5 Subtype AC (MUC5AC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 5 Subtype AC (MUC5AC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mucin 5 Subtype AC (MUC5AC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Pancreatic cyst fluid and serum mucin levels predict dysplasia in intraductal papillary mucinous neoplasms of the pancreas.; Naringin attenuates EGF-induced MUC5AC secretion in A549 cells by suppressing the cooperative activities of MAPKs-AP-1 and IKKs-IκB-NF-κB signaling pathways; Pyocyanin-induced mucin production is associated with redox modification of FOXA2; A novel serum marker for biliary tract cancer: Diagnostic and prognostic values of quantitative evaluation of serum mucin 5AC (MUC5AC); The human cathelicidin LL-37 enhances airway mucus production in chronic obstructive pulmonary disease; Proteomic analysis of cancer and mesothelial cells reveals an increase in Mucin 5AC during ovarian cancer and peritoneal interaction; The human Cathelicidin LL-37 induces MUC5AC mucin production by airway epithelial cells via TACE-TGF-α-EGFR pathway; Assessment of bile and serum mucin5AC in cholangiocarcinoma: Diagnostic performance and biologic significance; Leukotriene E 4 induces MUC5AC release from human airway epithelial NCI-H292 cells; Secreted mucins and airway bacterial colonization in non-CF bronchiectasis; Mucin Secretion In Asthmatics With Osmotic Airway Hyperresponsiveness; ReCavia (Guinea pig )lation of Viral Infection-induced Airway Remodeling Cytokine Production by the TLR3-EGFR Signaling Pathway in Human Bronchial Epithelial Cells; The influence of long-term corneal contact lens wearing on the stability and quality of tear film in Qinghai; Airway Mucin 2 Is Decreased in Patients with Severe Chronic Obstructive Pulmonary Disease with Bacterial Colonization; Impact of Cigarette Smoking on Tear Function and Correlation between Conjunctival Goblet Cells and Tear MUC5AC Concentration in Office Workers; Interleukin-33 induces mucin gene expression and goblet cell hyperplasia in human nasalepithelial cells.; Clarithromycin inhibits TNF-α-induced MUC5AC mucin gene expression viathe MKP-1-p38MAPK-dependent pathway; Секреторно-экссудативная реакция слизистой носа на воздействие холодного воздуха у больных бронхиальной астмой; An adherent mucus layer attenuates the genotoxic effect of colibactin.; Oxytetracycline Inhibits Mucus Secretion and Inflammation in Human Airway Epithelial Cells.; Salvianolic acid B improves airway hyperresponsiveness by inhibiting MUC5AC overproduction associated with Erk1/2/P38 signaling; Plantamajoside Inhibits Lipopolysaccharide-Induced MUC5AC Expression and Inflammation through Suppressing the PI3K/Akt and NF-κB Signaling Pathways in …; Profiling of healthy and asthmatic airway smooth muscle cells following IL-1β treatment: a novel role for CCL20 in chronic mucus hyper-secretion; Development of a highly sensitive and reliable enzyme-linked immunosorbent assay for MUC5aC in human tears extracted from schirmer strips; Increased expression of long non-coding RNA SNHG16 correlates with tumor progression and poor prognosis in non-small cell lung cancer; The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease; Regulation effects of naringin on diesel particulate matter-induced abnormal airway surface liquid secretion;