ELISA Kit for Osteocalcin (OC)

15.6-1, 000pg/mL

The minimum detectable dose of this kit is typically less than 6.3pg/mL

3h

This assay has high sensitivity and excellent specificity for detection of Osteocalcin (OC).
No significant cross-reactivity or interference between Osteocalcin (OC) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteocalcin (OC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Osteocalcin (OC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Osteocalcin (OC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Osteocalcin (OC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Biochemical study of the influence of ad type biophytomodulators in healing of bone defects in rats.; Oxytocin promotes bone formation during the alveolar healing process in old acyclic female rats; Exendin-4 Increases Bone Mineral Density in Type 2 Diabetic OLETF Rats Potentially Through the Down-Regulation of SOST/Sclerostin in Osteocytes; Serum changes induced by intramedullar experimental administration of bisphosphonates; Effect of the “protein diet” and bone tissue.; Treatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory Conditions; Treatment with α-lipoic acid enhances the bone healing after femoral fracture model of rats; Immobilizing osteogenic growth peptide with and without fibronectin on a titanium surface: effects of loading methods on mesenchymal stem cell differentiation; Transplantation of mesenchymal stem cells, recombinant human BMP-2, and their combination in accelerating the union after osteotomy and increasing, the mechanical strength of extracorporeally irradiated femoral autograft in rat models; The influence of bexarotene, a selective agonist of the retinoid receptor X (RXR), and tazarotene, a selective agonist of the retinoid acid receptor (RAR), on bone metabolism in rats; Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway; Antiosteoporotic effect of icariin in ovariectomized rats is mediated via the Wnt/β-catenin pathway; Impact of Curcumin and Flaxseed Oil on Bone Turnover in Glucocorticoid-Injected Female Rats; The effects of strength training and raloxifene on bone health in aging ovariectomized rats; Effect of Sodium Fluoride on Bone Biomechanical and Histomorphometric Parameters and on Insulin Signalingand Insulin Sensitivity in Ovariectomized Rats.; Protective Effect of Soybean and Mushroom against Glucocorticoid-Induced Osteoporosis in Female Rats; The controlled release of simvastatin from TiO2 nanotubes to promote osteoblast differentiation and inhibit osteoclast resorption; Noni leaf and black tea enhance bone regeneration in estrogen-deficient rats.; Ginsenosides Rg3 attenuates glucocorticoid-induced osteoporosis through regulating BMP-2/BMPR1A/Runx2 signaling pathway; Effects of long-term administration of pantoprazole on bone mineral density in young male rats.; Effects of fluoride on insulin signaling and bone metabolism in ovariectomized rats.; Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats; Oral administration of kaempferol inhibits bone loss in rat model of ovariectomy-induced osteopenia; A Water Extract of Benefiting-Bone Capsule Improves Osteoporosis via Transcriptional and Translational Regulation of Key Factors in Notch and Wnt/ò-catenin Signaling Pathways in Ovariectomized Rats; BMP-2 gene transfection of bone marrow stromal cells to induce osteoblastic differentiation in a rat calvarial defect model; Effect of long-term administration of mangiferin from on bone metabolism in ovariectomized rats; Effects of water extract from epimedium on neuropeptide signaling in an ovariectomized osteoporosis rat model; Myricetin ameliorates glucocorticoid-induced osteoporosis through the ERK signaling pathway; Combined effect of pulsed electromagnetic fields and narrowband ultraviolet B on bone metabolism in glucocorticoid-treated rats; In Situ Self-Assembly of Graphene Oxide/Polydopamine/Sr2+ Nanosheets on Titanium Surfaces for Enhanced Osteogenic Differentiation of Mesenchymal Stem Cells; The decline of whole-body glucose metabolism in ovariectomized rats; Mollugin attenuates glucocorticoid-induced osteoporosis in rats via Akt/P13K pathway; Influence of physical training on markers of bone turnover, mechanical properties, morphological alterations, density and mineral contents in the femur of rats exposed …; Methylbenzoxime as a therapeutic agent for glucocorticoid-induced osteoporosis in rats.; Preventive effect of dried plum extract against dexamethasone-induced osteoporosis in male rats through inhibiting cathepsin-K activity, lipogenesis and …;