ELISA Kit for Interleukin 8 (IL8)

15.6-1, 000pg/mL

The minimum detectable dose of this kit is typically less than 5.9pg/mL

3h

This assay has high sensitivity and excellent specificity for detection of Interleukin 8 (IL8).
No significant cross-reactivity or interference between Interleukin 8 (IL8) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 8 (IL8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 8 (IL8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 8 (IL8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 8 (IL8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Serial cytokine levels during wound healing in rabbit maxillary sinus mucosa; Experimental peri-implant mucositis at different implant surfaces.; Serum levels of selected chemokines in systemic lupus erythematosus patients; Expression of interleukine-8 as an independent prognostic factor for sporadic colon cancer dissemination; Effects of different ventilation strategies on exhaled nitric oxide in geriatric abdominal surgery; The Predictive Diagnostic and Prognostic Cut-off Values for Interleukin 8 in Patients with Meningitis in Egypt; Neutrophil gene dynamics and plasma cytokine levels in dairy cattle during peri-implantation period; Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol; Mutant DD genotype of NFKB1 gene is associated with the susceptibility and severity of coronary artery disease.; 小剂量阿奇霉素对稳定期老年慢性阻塞性肺疾病患者外周血内TNF-α、IL-8、CRP水平及肺功能的影响; Increased Chondrogenic Potential of Mesenchymal Cells From Adipose Tissue Versus Bone Marrow‐Derived Cells in Osteoarthritic In Vitro Models; The effect of restorative materials on cytokines in gingival crevicular fluid; Experimentelle periimplantäre Mukositis an unterschiedlichen Implantatoberflächen; CagA promotes proliferation and inhibits apoptosis of GES-1 cells by upregulating TRAF1/4-1BB; Optimization of Storage Temperature for Retention of Undifferentiated Cell Character of Cultured Human Epidermal Cell Sheets; Inhibitory Effect of Low-Intensity Pulsed Ultrasound on the Expression of Lipopolysaccharide-Induced Inflammatory Factors in U937 Cells.; Association Between Endothelial Progenitor Cells and Treatment Response in Non-Squamous Non-small Cell Lung Cancer Treated with Bevacizumab.; Inflammatory biomarkers and radiologic measurements in never-smokers with COPD: A cross-sectional study from the CODA cohort.; Polar extract of protects cartilage homeostasis: possible mechanism of action; Pathogen-dependent modulation of milk neutrophils competence, plasma inflammatory cytokines and milk quality during intramammary infection of Sahiwal (Bos …; Rho Kinase Type 1 (ROCK1) Promotes Lipopolysaccharide-induced Inflammation in Corneal Epithelial Cells by Activating Toll-Like Receptor 4 (TLR4) …; N-Acetylcysteine inhalation improves pulmonary function in patients received Liver Transplantation.; The characteristics and clinical significance of mucin levels in bronchoalveolar lavage fluid of patients with interstitial lung disease; Altered serum levels of type I collagen turnover indicators accompanied by IL-6 and IL-8 release in stable COPD; Chandrasekaran Chinampudur Velusami, Edwin Jothie Richard &; Multiple myeloma-derived exosomes are enriched of amphiregulin (AREG) and activate the epidermal growth factor pathway in the bone microenvironment …;