Vergleich

CLIA Kit for Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1)

ArtNr SCP853Hu-24T
Hersteller Cloud-Clone
Menge 24T
Kategorie
Typ Clia
Specific against Human
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
Alias NB; DAN; NO3; DAND1; Differential Screening-Selected Gene Aberrant In Neuroblastoma; DAN domain family member 1
Lieferbar
Quantity options
Detection range
27.4-20, 000pg/mL
Organism species
Homo sapiens (Human)
Sensitivity
The minimum detectable dose of this kit is typically less than 12.3pg/mL
Sample type
Serum, plasma, tissue homogenates and other biological fluids
Assay length
2h, 40min
Method
Double-antibody Sandwich
Specificity

This assay has high sensitivity and excellent specificity for detection of Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1).
No significant cross-reactivity or interference between Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 100µ L Substrate Solution. Incubate 10 minutes at 37° C;
8. Read RLU value immediately.
Test principle
The microplate provided in this kit has been pre-coated with an antibody specific to Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Neuroblastoma, Suppression Of Tumorigenicity 1 (NBL1) level in the sample or standard.;
Research Area
Tumor immunity;

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 24T
Lieferbar: In stock
lieferbar

Vergleichen

Auf den Wunschzettel

Angebot anfordern

Lieferzeit anfragen

Technische Frage stellen

Bulk-Anfrage stellen

Fragen zum Produkt?
 
Schließen