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CLIA Kit for Cardiotrophin Like Cytokine Factor 1 (CLCF1)

ArtNr SCC389Ra-24T
Hersteller Cloud-Clone
Menge 24T
Kategorie
Typ Clia
Specific against Rat
ECLASS 10.1 32161000
ECLASS 11.0 32161000
UNSPSC 41116126
Alias CLC; NR6; BSF3; CISS2; NNT1; Novel Neurotrophin-1; B Cell-Stimulating Factor-3
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Detection range
2.74-2, 000pg/mL
Organism species
Rattus norvegicus (Rat)
Sensitivity
The minimum detectable dose of this kit is typically less than 1.18pg/mL
Sample type
Serum, plasma and other biological fluids
Assay length
2h, 40min
Method
Double-antibody Sandwich
Specificity

This assay has high sensitivity and excellent specificity for detection of Cardiotrophin Like Cytokine Factor 1 (CLCF1).
No significant cross-reactivity or interference between Cardiotrophin Like Cytokine Factor 1 (CLCF1) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cardiotrophin Like Cytokine Factor 1 (CLCF1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cardiotrophin Like Cytokine Factor 1 (CLCF1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 100µ L Substrate Solution. Incubate 10 minutes at 37° C;
8. Read RLU value immediately.
Test principle
The microplate provided in this kit has been pre-coated with an antibody specific to Cardiotrophin Like Cytokine Factor 1 (CLCF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cardiotrophin Like Cytokine Factor 1 (CLCF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cardiotrophin Like Cytokine Factor 1 (CLCF1) level in the sample or standard.;
Research Area
Cytokine; Cardiovascular biology; Neuro science;

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 24T
Lieferbar: In stock
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