CLIA Kit for Pigment Epithelium Derived Factor (PEDF)

0.14-100ng/mL

The minimum detectable dose of this kit is typically less than 0.05ng/mL

2h, 40min

This assay has high sensitivity and excellent specificity for detection of Pigment Epithelium Derived Factor (PEDF).
No significant cross-reactivity or interference between Pigment Epithelium Derived Factor (PEDF) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The microplate provided in this kit has been pre-coated with an antibody specific to Pigment Epithelium Derived Factor (PEDF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Pigment Epithelium Derived Factor (PEDF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Pigment Epithelium Derived Factor (PEDF) level in the sample or standard.;