CLIA Kit for Apoptotic Peptidase Activating Factor 1 (APAF1)

13.7-10, 000pg/mL

The minimum detectable dose of this kit is typically less than 6.2pg/mL

2h, 40min

This assay has high sensitivity and excellent specificity for detection of Apoptotic Peptidase Activating Factor 1 (APAF1).
No significant cross-reactivity or interference between Apoptotic Peptidase Activating Factor 1 (APAF1) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The microplate provided in this kit has been pre-coated with an antibody specific to Apoptotic Peptidase Activating Factor 1 (APAF1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Apoptotic Peptidase Activating Factor 1 (APAF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Apoptotic Peptidase Activating Factor 1 (APAF1) level in the sample or standard.;