« Zurück
Home /
CLIA Kit for Lactoferrin (LTF)

CLIA Kit for Lactoferrin (LTF)

Hersteller	Cloud-Clone
Kategorie	ELISA & Immunoassays Immunoassay CLIA Kits CLIA
Typ	Clia
Specific against	Cattle
Menge	24T
ArtNr	SCA780Bo-24T
eClass 6.1	27150207
eClass 9.0	27150207
Lieferbar
Alias	HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1
Quantity options
Detection range	0.41-300ng/mL
Organism species	Bos taurus; Bovine (Cattle)
Sensitivity	The minimum detectable dose of this kit is typically less than 0.14ng/mL
Sample type	Serum, plasma, tissue homogenates, cell lysates, saliva, milk, cell culture supernates and other biological fluids
Assay length	2h, 40min
Method	Double-antibody Sandwich
Specificity	This assay has high sensitivity and excellent specificity for detection of Lactoferrin (LTF). No significant cross-reactivity or interference between Lactoferrin (LTF) and analogues was observed.
Precision	Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lactoferrin (LTF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lactoferrin (LTF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
Stability	The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary	1. Prepare all reagents, samples and standards; 2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C; 3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C; 4. Aspirate and wash 3 times; 5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C; 6. Aspirate and wash 5 times; 7. Add 100µ L Substrate Solution. Incubate 10 minutes at 37° C; 8. Read RLU value immediately.
Test principle	The microplate provided in this kit has been pre-coated with an antibody specific to Lactoferrin (LTF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Lactoferrin (LTF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Lactoferrin (LTF) level in the sample or standard.;
Research Area	Tumor immunity; Infection immunity;

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.