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CLIA Kit for Intercellular Adhesion Molecule 1 (ICAM1)

ArtNr SCA548Mu-5x96T
Hersteller Cloud-Clone
Menge 5x96 T
Quantity options 10x96 T 24 T 48 T 5x96 T 96 T
Kategorie
Typ Elisa-Kit
Applikationen IA
Specific against Mouse (Murine, Mus musculus)
Sensitivity The minimum detectable dose of this kit is typically less than 29.2pg/mL
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias CD54; BB2; P3.58; Human Rhinovirus Receptor; Major group rhinovirus receptor
Lieferbar
Specificity Mus musculus (Mouse)
Quantity options
Detection range
68.6-50, 000pg/mL
Organism species
Mus musculus (Mouse)
Sensitivity
The minimum detectable dose of this kit is typically less than 29.2pg/mL
Sample type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length
2h, 40min
Method
Double-antibody Sandwich
Specificity

This assay has high sensitivity and excellent specificity for detection of Intercellular Adhesion Molecule 1 (ICAM1).
No significant cross-reactivity or interference between Intercellular Adhesion Molecule 1 (ICAM1) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Intercellular Adhesion Molecule 1 (ICAM1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µ L standard or sample to each well. Incubate 2 hours at 37° C;
3. Aspirate and add 100µ L prepared Detection Reagent A. Incubate 1 hour at 37° C;
4. Aspirate and wash 3 times;
5. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
6. Aspirate and wash 5 times;
7. Add 100µ L Substrate Solution. Incubate 10 minutes at 37° C;
8. Read RLU value immediately.
Test principle
The microplate provided in this kit has been pre-coated with an antibody specific to Intercellular Adhesion Molecule 1 (ICAM1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Intercellular Adhesion Molecule 1 (ICAM1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Intercellular Adhesion Molecule 1 (ICAM1) level in the sample or standard.;
Research Area
CD & Adhesion molecule; Tumor immunity; Infection immunity;

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 5x96 T
Lieferbar: In stock
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Lieferung vsl. bis 01.05.2025 

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