High Sensitive ELISA Kit for Cardiac Troponin I (cTnI)

3.12-200pg/mL

The minimum detectable dose of this kit is typically less than 1.29pg/mL

3h

This assay has high sensitivity and excellent specificity for detection of High Sensitive Cardiac Troponin I (cTnI).
No significant cross-reactivity or interference between High Sensitive Cardiac Troponin I (cTnI) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Cardiac Troponin I (cTnI) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Cardiac Troponin I (cTnI) and unlabeled High Sensitive Cardiac Troponin I (cTnI) (Standards or samples) with the pre-coated antibody specific to High Sensitive Cardiac Troponin I (cTnI). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Cardiac Troponin I (cTnI) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Cardiac Troponin I (cTnI) in the sample.