ArtNr |
CEA833Ra-10x96T |
Hersteller |
Cloud-Clone
|
Menge |
10x96T |
Kategorie |
|
Typ |
Elisa-Kit |
Specific against |
Rat |
ECLASS 10.1 |
32160605 |
ECLASS 11.0 |
32160605 |
UNSPSC |
41116126 |
Alias |
F8VWF, VWD, von Willebrand antigen 2 |
Lieferbar |
|
Quantity options |
|
Detection range |
12.35-1, 000ng/mL |
Organism species |
Rattus norvegicus (Rat) |
Sensitivity |
The minimum detectable dose of this kit is typically less than 4.99ng/mL |
Sample type |
Plasma |
Assay length |
2h |
Method |
Competitive Inhibition |
Specificity |
This assay has high sensitivity and excellent specificity for detection of Von Willebrand Factor (vWF). No significant cross-reactivity or interference between Von Willebrand Factor (vWF) and analogues was observed. |
Precision |
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Von Willebrand Factor (vWF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Von Willebrand Factor (vWF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% |
Stability |
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
Assay procedure summary |
1. Prepare all reagents, samples and standards; 2. Add 50µ L standard or sample to each well. And then add 50µ L prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37° C; 3. Aspirate and wash 3 times; 4. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C; 5. Aspirate and wash 5 times; 6. Add 90µ L Substrate Solution. Incubate 10-20 minutes at 37° C; 7. Add 50µ L Stop Solution. Read at 450 nm immediately. |
Test principle |
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Von Willebrand Factor (vWF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Von Willebrand Factor (vWF) and unlabeled Von Willebrand Factor (vWF) (Standards or samples) with the pre-coated antibody specific to Von Willebrand Factor (vWF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Von Willebrand Factor (vWF) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Von Willebrand Factor (vWF) in the sample. |
Research Area |
Signal transduction; Hematology; |
Reference |
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