ELISA Kit for Testosterone (Testo)

49.4-4, 000pg/mL

The minimum detectable dose of this kit is typically less than 17.7pg/mL

2h

This assay has high sensitivity and excellent specificity for detection of Testosterone (Testo).
No significant cross-reactivity or interference between Testosterone (Testo) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Testosterone (Testo) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Testosterone (Testo) and unlabeled Testosterone (Testo) (Standards or samples) with the pre-coated antibody specific to Testosterone (Testo). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Testosterone (Testo) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Testosterone (Testo) in the sample.

Androgen Deprivation Induces Bladder Histological Abnormalities and Dysfunction via TGF-β in Orchiectomized Mature Rats; Insulin Receptor Substrate 2 Is Required for Testicular Development; Central effects of camphor on GnRH and sexual hormones in male rat; Effects of Tadalafil on Hemorrhagic Cystitis and Testicular Dysfunction Induced by Cyclophosphamide in Rats; The effect of sertraline, paroxetine, fluoxetine and escitalopram on testicular tissue and oxidative stress parameters in rats.; Nanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineering; Critical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesis; Diets rich in saturated and polyunsaturated fatty acids induce morphological alterations in the rat ventral prostate; Carbamazepine-exposure during gestation and lactation affects pubertal onset and spermatic parameters in male pubertal offspring; 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Elevated Serum Bisphenol A Level in Patients with Dilated Cardiomyopathy; Effect on the H20 gene methylation of sperm and organs of offspring after chlorpyrifos‐methyl exposure during organogenesis period; Testosterone replacement attenuates mitochondrial damage in a rat model of myocardial infarction; Preparation and evaluation of a phospholipid-based injectable gel for the long term delivery of leuprolide acetaterrh; Association between Polycystic Ovary Syndrome and Cavia (Guinea pig )t Microbiota; Expression of androgen-producing enzyme genes and testosterone concentration in AnCavia (Guinea pig )s and Nellore heifers with high and low ovarian follicle count; Testosterone Replacement Modulates Cardiac Metabolic Remodeling after Myocardial Infarction byUpreCavia (Guinea pig )lating PPARα; THE EFFECT OF KISSPEPTIN ON SPERMATOGENESIS AND APOPTOSIS IN RATS; Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice; Molecular characterization of insulin resistance and glycolytic metabolism in the rat uterus; 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The effect of testosterone supplementation on morphometry and VEGF expression level of thoracic aorta in aged mice; Impaired steroidogenesis in the testis of leptin-deficient mice (ob/ob -/-); Beneficial effects of Heqi san on rat model of polycystic ovary syndrome through the PI3K/AKT pathway; Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population; Exposure to tris (1, 3-dichloro-2-propyl) phosphate for Two generations decreases fecundity of zebrafish at environmentally relevant concentrations; deficiency leads to testicular dysfunction and altered expression of genes involved in cell communication, chromatin reorganization, spermatogenesis, and …; DHEA-induced ovarian hyperfibrosis is mediated by TGF-β signaling pathway; Eicosapentaenoic Acid Improves Polycystic Ovary Syndrome in Rats via Sterol Regulatory Element-Binding Protein 1 (SREBP-1)/Toll-Like Receptor 4 (TLR4) …; Prostaglandin E2 triggers cytochrome P450 17α hydroxylase overexpression via signal transducer and activator of transcription 3 phosphorylation and promotes …; Crocetin attenuates DHT-induced polycystic ovary syndrome in mice via revising kisspeptin neurons; Resveratrol attenuates metabolic, sperm, and testicular changes in adult Wistar rats fed a diet rich in lipids and simple carbohydrates; 基因敲除损伤小鼠睾丸睾酮产生与生精功能; Protective Effects of Uncultured Adipose‐Derived Stromal Vascular Fraction on Testicular Injury Induced by Torsion‐Detorsion in Rats; Demographic, clinical and lifestyle predictors for severity of erectile dysfunction and biomarkers level in Malaysian patients; Uterine progesterone signaling is a target for metformin therapy in polycystic ovary syndrome; Brain‐derived neurotrophic factor: A steroidogenic regulator of Leydig cells; Tanshinone IIA attenuates estradiol‑induced polycystic ovarian syndrome in mice by ameliorating FSHR expression in the ovary; iTRAQ-Based Proteomics to Reveal the Mechanism of Hypothalamus in Kidney-Yin Deficiency Rats Induced by Levothyroxine; The therapeutic potential of amifostine on cyclophosphamide-induced testicular dysfunction in rats: An experimental study.; The Effect of Testosterone Supplementation on Capillar Morphometry and VEGF Expression Level of the Heart in Aged Mice; Cyclophilin D participates in the inhibitory effect of high‐fat diet on the expression of steroidogenic acute regulatory protein; Sperm parameters quality and reproductive effects of methanolic extract of Alchornea cordifolia leaves on senescent male rats;