ELISA Kit for Glutathione (GSH)

1.23-100ug/mL

The minimum detectable dose of this kit is typically less than 0.52ug/mL

2h

This assay has high sensitivity and excellent specificity for detection of Glutathione (GSH).
No significant cross-reactivity or interference between Glutathione (GSH) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Glutathione (GSH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Glutathione (GSH) and unlabeled Glutathione (GSH) (Standards or samples) with the pre-coated antibody specific to Glutathione (GSH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Glutathione (GSH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Glutathione (GSH) in the sample.

Evidence for augmented oxidative stress in the subjects with type 1 diabetes and their siblings: a possible preventive role for antioxidants; Glutathione S-Transferase P1 Correlated with Oxidative Stress in Hepatocellular Carcinoma; Alteration in the intrafollicular thiol–redox system in infertile women with endometriosis; Effects of mild hypothermia therapy on the levels of glutathione in rabbit blood and cerebrospinal fluid after cardiopulmonary resuscitation; Oxidative Stability of Ostrich Meat Related to Duration of Linseed and Lucerne Supplementation to the Bird' s Diet; Prenatal maternal distress affects atopic dermatitis in offspring mediated by oxidative stress; Hydrogen sulfide protects against chronic unpredictable mild stress-induced oxidative stress in hippocampus by upreCavia (Guinea pig )lation of BDNF-TrkB pathway; Rapid opioid detoxification with an implementation of the new method of gradually increasing dosage of naltrexone induction; Toxicity study of oxalicumone A, derived from a marine-derived fungus Penicillium oxalicum, in cultured renal epithelial cells; Hepatocellular toxicity of oxalicumone A via oxidative stress injury and mitochondrial dysfunction in healthy human liver cells; Metabolomic and transcriptomic profiling of hepatocellular carcinomas in Hras12V transgenic mice; Circulating cell-free DNA of methylated insulin-like growth factor-binding protein 7 predicts a poor prognosis in hepatitis B virus-associated hepatocellular carcinoma …; Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling Pathway; Epigallocatechingallate attenuates myocardial injury in a mouse model of heart failure through TGF‑β1/Smad3 signaling pathway; Effects of ginsenoside Rb1 on oxidative stress injury in rat spinal cords by regulating the eNOS/Nrf2/HO‑1 signaling pathway; Effects of Tualang honey in modulating nociceptive responses at the spinal cord in offspring of prenatally stressed rats; Protective effects of sulforaphane on diabetic retinopathy: activation of the Nrf2 pathway and inhibition of NLRP3 inflammasome formation; Porous Se@ SiO2 nanosphere-coated catheter accelerates prostatic urethra wound healing by modulating macrophage polarization through reactive oxygen species …; MicroRNA‑21 contributes to the puerarin‑induced cardioprotection via suppression of apoptosis and oxidative stress in a cell model of ischemia/reperfusion injury; Germinated-soy milk as a healthy diet to induce high antioxidant enzymes in breast milk;