CLIA Kit for Estriol (E3)

781-200, 000pg/mL

The minimum detectable dose of this kit is typically less than 262.3pg/mL

2h

This assay has high sensitivity and excellent specificity for detection of Estriol (E3).
No significant cross-reactivity or interference between Estriol (E3) and analogues was observed.

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Estriol (E3). A competitive inhibition reaction is launched between biotin labeled Estriol (E3) and unlabeled Estriol (E3) (Standards or samples) with the pre-coated antibody specific to Estriol (E3). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Estriol (E3) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Estriol (E3) level in the sample or standard.