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CLIA Kit for Angiotensin II (AngII)

ArtNr CCA005Hu-5x96T
Hersteller Cloud-Clone
Menge 5x96 T
Quantity options 10x96 T 24 T 48 T 5x96 T 96 T
Kategorie
Typ Elisa-Kit
Applikationen IA
Specific against Human (Homo sapiens)
Sensitivity The minimum detectable dose of this kit is typically less than 3.61pg/mL
Citations T lymphocyte-based renin angiotensin system in obesity
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias Ang-II; Angiotensin-2
Lieferbar
Specificity Homo sapiens (Human)
Quantity options
Detection range
11.72-3, 000pg/mL
Organism species
Homo sapiens (Human)
Sensitivity
The minimum detectable dose of this kit is typically less than 3.61pg/mL
Sample type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay length
2h
Method
Competitive Inhibition
Specificity

This assay has high sensitivity and excellent specificity for detection of Angiotensin II (AngII).
No significant cross-reactivity or interference between Angiotensin II (AngII) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin II (AngII) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin II (AngII) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 50µ L standard or sample to each well.
        And then add 50µ L prepared Detection Reagent A immediately.
        Shake and mix. Incubate 1 hour at 37° C;
3. Aspirate and wash 3 times;
4. Add 100µ L prepared Detection Reagent B. Incubate 30 minutes at 37° C;
5. Aspirate and wash 5 times;
6. Add 100µ L Substrate Solution. Incubate 10 minutes at 37° C;
7. Read RLU value immediately.
Test principle
The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Angiotensin II (AngII). A competitive inhibition reaction is launched between biotin labeled Angiotensin II (AngII) and unlabeled Angiotensin II (AngII) (Standards or samples) with the pre-coated antibody specific to Angiotensin II (AngII). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Angiotensin II (AngII) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Angiotensin II (AngII) level in the sample or standard.
Research Area
Endocrinology; Cardiovascular biology;
Reference

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 5x96 T
Lieferbar: In stock
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