Rat IGFBP-4 ELISA Kit

Rat IGFBP4 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat IGFBP4 in serum, plasma, tissue homogenates, and other biological fluids.

IGFBP4

Rat IGFBP4 ELISA Kit (A75510) employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat IGFBP4 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for IGFBP4 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the IGFBP4 present in each sample is bound to the wells by the immobilized antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-IGFBP4 Antibody, which binds the captured IGFBP4 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of IGFBP4 captured in each well. The concentration of IGFBP4 can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.

Colorimetric

4h 30m