Mouse TNF-α ELISA Kit

This assay has shown no significant cross-reactivity.

Store at 2 - 8°C.

Mouse TNF-α ELISA Kit is based on the quantitative sandwich enzyme-linked immunosorbent assay technique to measure concentration of mouse TNF-α in the samples. A monoclonal antibody specific for mouse TNF-α has been immobilized onto microwells. Standard or samples are pipetted into the wells, followed by the addition of biotin-linked monoclonal antibody specific for TNF-α, and TNF-α present is bound by the immobilized antibody and detect antibody following the first incubation. After removal of any unbound substances, streptavidin-HRP is added for a second incubation. After washing, substrate solution reacts with HRP and color develops in proportion to the amount of TNF-α bound by the immobilized antibody. The color development is stopped by addition of acid and the optical density value is measured by microplate reader.

Colorimetric

99% (Average Recovery from Serum Samples).