ArtNr |
ELS-PKSM040864-100ug |
Hersteller |
Elabscience
|
Menge |
100ug |
Kategorie |
|
Typ |
Proteins |
Specific against |
Mouse |
Host |
HEK293 cells |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
T-cell-specific surface glycoprotein CD28,CD28,CH29-189K17.3 |
Similar products |
CD28, T-cell-specific surface glycoprotein CD28, CH29-189K17.3 |
Lieferbar |
|
Synonym |
T-cell-specific surface glycoprotein CD28, CD28, CH29-189K17.3 |
Storage |
according to manual |
Activity |
Measured by its binding ability in a functional ELISA.1. Immobilized mouse CD86 at 20 ug/ml (100 ul/well) can bind mouse CD28 Fc Chimera with a linear ranger of 6.4-800 ng/ml.2. Immobilized rat CD86 at 2 ug/ml (100 ul/well) can bind mouse CD28 Fc Chimera with a linear ranger of 6.4-160 ng/ml. |
Protein Construction |
A DNA sequence encoding the extracellular domain (Met 1-Lys 149) of mouse CD28 (NP_031668.3) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus. |
Sequence |
Met 1-Lys 149 |
Fusion tag |
C-His-Fc |
Accession |
NP_031668.3 |
Shipping |
This product is provided as lyophilized powder which is shipped with ice packs. |
Purity |
> 96 % as determined by SDS-PAGE |
Endotoxin |
< 1.0 EU per ug of the protein as determined by the LAL method. |
Stability and Storage |
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80CC. Reconstituted protein solution can be stored at 4-8C for 2-7 days. Aliquots of reconstituted samples are stable at < -20C for 3 months. |
Mol Mass |
43 kDa |
AP Mol Mass |
55-60 kDa |
Formulation |
Lyophilized from sterile PBS, pH 7.4 |
Reconstitution |
Please refer to the printed manual for detailed information. |
Background |
CD28 (Cluster of Differentiation 28) is a disulphide-bonded glycoprotein belonging to the immunoglobulin (Ig) superfamily, and structurally consists of a single Ig V-like extracellular domain, a transmembrane domain and an intracellular domain. Mouse CD28 is constitutively expressed on the surface of all murine T cells and on developing thymocytes as disulfide-linked homodimers or as monomers. CD28 can binds the B7-1 and B7-2 ligand, and together perform important functions in the T and B cell response pathways. B7/CD28 family members, which can augment or antagonize T-cell receptor signaling, in the regulation of central and peripheral T-cell tolerance. CD28 is thus involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.Immune CheckpointImmune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IHC Antibodies Immune Checkpoint Detection: FCM Antibodies Immune Checkpoint Detection: WB AntibodiesImmune Checkpoint Targets Co-stimulatory Immune Checkpoint Targets Immunotherapy Cancer Immunotherapy Targeted Therapy |
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