ArtNr |
ELS-PKSH031808-100ug |
Hersteller |
Elabscience
|
Menge |
100ug |
Kategorie |
|
Typ |
Proteins |
Specific against |
Human |
Host |
HEK293 cells |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
D1S1733E,DAN,DAND1,NB,NO3 |
Similar products |
D1S1733E, DAN, DAND1, NB, NO3 |
Lieferbar |
|
Synonym |
D1S1733E, DAN, DAND1, NB, NO3 |
Storage |
according to manual |
Activity |
Measured by its ability to inhibit BMP4-induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 5-20 ug/ml in the presence of 50 ng/mL of recombinant human BMP4. |
Protein Construction |
A DNA sequence encoding the human DAN precursor (NP_005371.1) (Met 1-Asp 180) was expressed with a C-terminal polyhistidine tag. |
Sequence |
Met 1-Asp 180 |
Fusion tag |
C-His |
Accession |
NP_005371.1 |
Shipping |
This product is provided as lyophilized powder which is shipped with ice packs. |
Purity |
> 98 % as determined by reducing SDS-PAGE. |
Endotoxin |
< 1.0 EU per ug as determined by the LAL method. |
Stability and Storage |
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80CC. Reconstituted protein solution can be stored at 4-8C for 2-7 days. Aliquots of reconstituted samples are stable at < -20C for 3 months. |
Mol Mass |
19.3 kDa |
AP Mol Mass |
27 kDa |
Formulation |
Lyophilized from sterile PBS; pH 7.4 |
Reconstitution |
Please refer to the printed manual for detailed information. |
Background |
The Dan (Differential screening-selected gene aberrative in neuroblastoma; also known as N03) gene was first identified as the putative rat tumor suppressor gene and encodes a protein structurally related to Cerberus and Gremlin in vertebrates. It is a founding member of the DAN family of secreted proteins; acts as an inhibitor of cell cycle progression and is closely involved in retinoic acid-induced neuroblastoma differentiation. There are at least five mammalian protein members in the evolutionarily conserved Dan family including DAN; Gremlin/DRM; Cer1 (Cerberus-related); Dante and PRDC (protein related to DAN and cereberus); and share the C-terminal cystine-knot motif. As a secreted glycoprotein; DAN is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways. It binds to BMPs and preventing their interactions with signaling receptor complexes; and accordingly regulates the processes of embryonic development and tissue differentiation. DAN gene product may have an important role in regulation of the entry of cells into the S phase. In addition; DAN gene product possesses an ability to revert phenotypes of transformed rat fibroblasts and represents a candidate tumour suppressor gene for neuroblastoma. |
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