Recombinant Human NBL1/DAND1 Protein (His Tag)(Active)

according to manual

A DNA sequence encoding the human DAN precursor (NP_005371.1) (Met 1-Asp 180) was expressed with a C-terminal polyhistidine tag.

C-His

This product is provided as lyophilized powder which is shipped with ice packs.

< 1.0 EU per ug as determined by the LAL method.

19.3 kDa

Lyophilized from sterile PBS; pH 7.4

The Dan (Differential screening-selected gene aberrative in neuroblastoma; also known as N03) gene was first identified as the putative rat tumor suppressor gene and encodes a protein structurally related to Cerberus and Gremlin in vertebrates. It is a founding member of the DAN family of secreted proteins; acts as an inhibitor of cell cycle progression and is closely involved in retinoic acid-induced neuroblastoma differentiation. There are at least five mammalian protein members in the evolutionarily conserved Dan family including DAN; Gremlin/DRM; Cer1 (Cerberus-related); Dante and PRDC (protein related to DAN and cereberus); and share the C-terminal cystine-knot motif. As a secreted glycoprotein; DAN is a member of a class of glycoproteins shown to be secreted inhibitors of the transforming growth factor-beta (TGF-beta) and bone morphogenic protein pathways. It binds to BMPs and preventing their interactions with signaling receptor complexes; and accordingly regulates the processes of embryonic development and tissue differentiation. DAN gene product may have an important role in regulation of the entry of cells into the S phase. In addition; DAN gene product possesses an ability to revert phenotypes of transformed rat fibroblasts and represents a candidate tumour suppressor gene for neuroblastoma.