Recombinant Human TGFBR2 Protein (His & Fc Tag)(Active)

AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TGFbeta-RII, TGFR-2, TGF-beta receptor type-2, TGF-beta type II receptor, TGFBR2, Transforming growth factor-beta receptor type II

Measured by its ability to inhibit TGF-beta1 activity on Mv-1-lu mink lung epithelial cells. The ED50 for this effect is typically 0.2-3.0 ug/ml in the presence of 1 ng/mL of recombinant human TGF-beta1.

Met 1-Asp 159

NP_003233.4

> 97 % as determined by reducing SDS-PAGE.

Lyophilized proteins are stable for up to 12 months when stored at -20 to -80CC. Reconstituted protein solution can be stored at 4-8C for 2-7 days. Aliquots of reconstituted samples are stable at < -20C for 3 months.

Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5

TGFBR2 is member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. It is a transmembrane protein. TGFBR2 is comprised by a C-terminal protein kinase domain and an N-terminal ectodomain. The ectodomain consists of a compact fold containing nine beta-strands and a single helix stabilised by a network of six intra strand disulphide bonds. The folding topology includes a central five-stranded antiparallel beta-sheet, eight-residues long at its centre, covered by a second layer consisting of two segments of two-stranded antiparallel beta-sheets. TGFBR2 has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in TGFBR2 gene have been associated with Marfan syndrome, Loeys-Deitz Aortic Aneurysm Syndrome, and the development of various types of tumors. TGFBR2 attenuates the biological activities of TGF-beta in colorectal cancer. TGFBR2 expression is increased in oral squamous cell carcinoma cells. Its expression is decreased by IL-1beta while inducing Sp3 via NFkappaB. TGFB2 and TGFBR2 are involved in the antiestrogenic activity.