H-Ras Protein; wild type 6xHis Tag

On Arrival: 4°C

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The human H-Ras protein has been produced in a bacterial expression system. The protein is supplied as a lyophilized powder.When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl₂ 0.5% sucrose, 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent, Cat. # ADV02.
The recombinant protein is approximately 28 kDa, consisting of the H-Ras protein plus a histidine tag in the amino-terminus.
For other forms of Ras as well as many other purified small G-proteins, see our main small G-protein product page.

Figure 1. His-H-Ras protein purity determination. A 10 ug sample of RS01 (His-H-Ras molecular weight approx. 28 kDa) was separated by electrophoresis in a 12% SDS-PAGE system. The protein was stained with Coomassie Blue.