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Raf protein ras binding domain on GST beads

Raf protein ras binding domain on GST beads

ArtNr	RF02-B
Hersteller	Cytoskeleton
Menge	6 x 2 mg
Quantity options	2 x 2 mg 6 x 2 mg
Kategorie	Oncology Neuroscience Targets for Rodent Neurosciences Neural Signaling By Cell Type Blastoma Carcinoma Glioma Hematology Lymphoma Myeloma Melanoma Sarcoma Cell Biology Cytoskeleton Reagents & Chemicals Astrocytoma Nanoparticles Beads & Resin By Organ Hepatic Cancer small GTPases Rho Subfamily
Typ	Proteins
Specific against	other
Purity	Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 12% SDS polyacrylamide gel. GST-Raf-RBD protein is >80% pure (see Figure 2).
Citations	Malilas et al., 2013. Cancer upregulated gene 2, a novel oncogene, confers resistance to oncolytic vesicular stomatitis virus through STAT1-OASL2 signaling. Cancer Gene Ther. doi:10.1038/cgt.2012.96. Takeishi et al., 2012. Diacylglycerol kinase alpha enhances hepatocellular carcinoma progression by activation of Ras&ndash,Raf&ndash,MEK&ndash,ERK pathway. J. Hepatol. 57, 77-83. Zhang et al., 2004. Protein kinase C (PKC) &beta,II induces cell invasion through a Ras/Mek-, PKC &iota,/Rac 1-dependent signaling pathway. J. Biol. Chem. 279, 22118-22123.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Small G protein, Raf, Raf-RBD, ras binding domain, c-Raf
Similar products	c-Raf, Raf, Small G protein, Raf-RBD, ras binding domain
Versandbedingung	Raumtemperatur
Lieferbar
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Delivery Time	1-2 Weeks
Weight (grams)	18
Product Uses	Measurement of the GTP/GDP ratio of Ras in vitro. Quantitation of GTP-Ras from tissue and tissue culture cell lysates.
Material	The Ras binding domain (RBD) of the c-Raf kinase protein (Raf) protein has been expressed as a GST-fusion protein in E. coli. This protein binds binds specifically to GTP-bound, and not GDP-bound, Ras proteins. The domain can therefore be used to specifically precipitate active, GTP-bound Ras as well as to specifically block the activity of Ras in vitro and in vivo. The protein is supplied in a glutathione agarose bound format and is shipped lyophilized. The beads are colored for ease of use. This product is used in our Ras activation assay Biochem Kit (Cat. # BK008). The GST-tagged Raf-RBD protein can be released from the beads by incubation with 10 mM reduced glutathione.
Biological Activity	Raf-RBD protein specifically recognizes and binds the active, GTP-bound, forms of Ras proteins. It has a much lower affinity for the inactive, GDP-bound, form Ras.When coupled to a colored glutathione sepharose matrix, the Raf-RBD protein beads become a convienent tool for assaying the activity of the Ras proteins. The quality control biological assay for Raf-RBD protein beads consists of a Ras protein pulldown frombovine brain extracts loaded with either GTP?S (Cat. # BS01) or GDP.
Figure 1 Legend	The brightly colored glutatione agarose beads in RF02 are easy to use.
Figure 2 Legend	Figure 2. GST-Raf-RBD protein purity determination. A 20 ug sample of RF02 was separated by electrophoresis in a 12% SDS-PAGE system and stained with Coomassie Blue. The GST-Raf-RBD protein runs at approximately 35 kDa.

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RF02-B.pdf

RF02-B-datasheet.pdf

RF02-B-safety-datasheet.pdf