Vergleich

Rac1 His Protein: wild type

ArtNr RC01-C
Hersteller Cytoskeleton
Menge 3 x 100 ug
Quantity options 1 x 100 ug 3 x 100 ug 1 x 1 mg
Kategorie
Typ Proteins
Specific against other
Purity Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. His-Rac1 samples are >90% pure.
Citations Kulkarni, S., Goll, D. E. and Fox, J. E. (2002). Calpain cleaves RhoA generating a dominant-negative form that inhibits integrin-induced actin filament assembly and cell spreading. J. Biol. Chem. 277, 24435-24441.
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Small G protein, Rac, Rac1, Rac 1, Rac1 protein
Similar products Rac, Rac1, Rac 1, Small G protein, Rac1 protein
Versandbedingung Raumtemperatur
Lieferbar
Shipping Temperature
AT
Storage Conditions
On Arrival: 4°C
Delivery Time
1-2 Weeks
Weight (grams)
21
Product Uses
Rac biochemistry
Rac1 GTPase assays
Rac1 nucleotide exchange assays
Rac1 binding studies
Material
The human Rac1 protein has been produced in a bacterial expression system. The protein is supplied as a lyophilized powder.When it is reconstituted in distilled water to 5 mg/ml, the protein is in the following buffer: 50 mM Tris pH 7.5, 0.5 mM MgCl2 50 mM NaCl, 0.5% sucrose, and 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent, Cat. # ADV02.
The recombinant protein is 22 kDa, consisting of the Rac1 protein plus a histidine tag in the amino-terminus.
For other forms of Rac1 as well as many other purified small G-proteins, see our main small G-protein product page
Biological Activity
The biological activity of RC01 is determined by its ability to exchange nucleotide. This is tested by a pulldown assay using GST-tagged PAK-PBD beads (Cat. # PAK02) and GTP?S (Cat. # BS01) or GDP loaded His-Rac1. The PAK protein is an effector of Rac1, and will specifically bind to the GTP bound form Rac1. Using this assay, the amount of biologically active GTP-bound Rac1 is determined. Stringent quality control ensures that > 80% of the His-Rac1 protein produced is capable of binding GTP.
Figure 1 Legend
Figure 1. His-Rac1 protein purity determination. A 10 ug sample of RC01 (His-Rac1 molecular weight approx. 22 kDa) was separated by electrophoresis in a 12% SDS-PAGE system. The protein was stained with Coomassie Blue.<

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 3 x 100 ug
Lieferbar: In stock
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