ArtNr |
CD01-C |
Hersteller |
Cytoskeleton
|
Menge |
3 x 100 ug |
Quantity options |
1 x 100 ug
3 x 100 ug
1 x 1 mg
|
Kategorie |
|
Typ |
Proteins |
Specific against |
other |
Purity |
Purity is determined by scanning densitometry of proteins on SDS-PAGE gels. His-Cdc42 samples are >90% pure. |
Citations |
Nevins, A. K. and Thurmond, D. C. (2005). A direct interaction between Cdc42 and vesicle-associated membrane protein 2 regulates SNARE-dependent insulin exocytosis. J. Biol. Chem. 280, 1944-1952. Kulkarni, S., Goll, D. E. and Fox, J. E. (2002). Calpain cleaves RhoA generating a dominant-negative form that inhibits integrin-induced actin filament assembly and cell spreading. J. Biol. Chem. 277, 24435-24441. |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Small G protein, Cdc42 protein, Cdc42, Cdc 42, Cdc42 GTPase assay, his-Cdc42 |
Similar products |
Cdc42, Cdc 42, Small G protein, Cdc42 protein, his-Cdc42, Cdc42 GTPase assay |
Versandbedingung |
Raumtemperatur |
Lieferbar |
|
Shipping Temperature |
AT |
Storage Conditions |
On Arrival: 4°C |
Delivery Time |
1-2 Weeks |
FAQs |
Question 1: After reconstituting the lyophilized protein with water, what is the composition of the buffer the wild-type human Cdc42 protein is in? Answer 1: The protein should be reconstituted to 5 mg/ml by the addition of Milli-Q water. The protein will be in the following buffer: 50 mM Tris pH 7.6, 0.5 mM MgCl2, 50 mM NaCl, 3% sucrose, and 0.6% dextran. In order to maintain high biological activity of the protein, it is strongly recommended that the protein solution be supplemented with DTT to 1 mM final concentration. Question 2: Can the wild-type human Cdc42 protein be microinjected into cells? Answer 2: Yes, the wild-type human Cdc42 protein can be microinjected into living cells to monitor the localization of Cdc42. |
Weight (grams) |
24 |
Product Uses |
Cdc42 biochemistry Cdc42 GTPase assays Cdc42 nucleotide exchange assays Cdc42 binding studies |
Material |
The human Cdc42 protein has been produced in a bacterial expression system. The protein is supplied as a lyophilized powder.When it is reconstituted in distilled water to 1 mg/ml, the protein is in the following buffer: 2 mM Tris pH 7.6, 0.5 mM MgCl 2 0.5% sucrose, 0.1% dextran. Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent, Cat. # ADV02. The recombinant protein is 22 kDa, consisting of the 22 kDa Cdc42 protein plus a 6 amino acid histidine tag in the amino-terminus. For other forms of Cdc42 as well as many other purified small G-proteins, see our main small G-protein product page |
Biological Activity |
The biological activity of CD01 is determined by its ability to exchange nucleotide. This is tested by a pulldown assay using GST-tagged PAK-1 PBD beads (Cat. # PAK02) and GTP?S (Cat. # BS01) or GDP loaded His-Cdc42. The PAK (p21 activated kinase) protein is an effector of Cdc42, and will specifically bind to the GTP bound form Cdc42. Using this assay, the amount of biologically active GTP-bound Cdc42 is determined. Stringent quality control ensures that > 70% of the Cdc42 protein produced is capable of binding GTP. |
Figure 1 Legend |
His-Cdc42 protein purity determination. A 10 ug sample of CD01 (His-Cdc42 molecular weight approx. 22 kDa) was separated by electrophoresis in a 12% SDS-PAGE system. The protein was stained with Coomassie Blue. |