Fibronectin Biotin (bovine plasma)

AT

1-2 Weeks

14

Fibronectin is purified from bovine plasma. Protein purity is determined by scanning densitometry of coomassie blue stained protein on a 4-20% polyacrylamide gel. Biotinylated fibronectin is > 80% pure (Figure 1).
The protein is modified to contain covalently linked biotins at random surface lysines. A long-chain activated ester of biotin biotin-XX, succinimidyl ester is used to label the protein. Labeling efficiency is determined by the ability to detect 10 ng biotinylated fibronectin using alkaline phosphatase conjugated streptavidin (Figure 2).
Fibronectin runs as individual subunits on SDS-PAGE with an apparent molecular weight of 230 kDa. FNR03 is supplied as a white lyophilized powder. Each vial contains 20 ug protein.

Legend: Serial dilutions of biotinylated fibronectin were separated by electrophoresis on a 4-20% polyacrylamide gel, blotted to PVDF, probed with a 1:1000 dilution of streptavidin alkaline phosphatase (Sigma) and detected with 1-Step NBT/BCIP reagent (Pierce). Lane 1, 100 ng, Lane 2, 50 ng, Lane 3, 40 ng, Lane 4, 20 ng, and Lane 5, 10 ng of biotinylated fibronectin. Lane M, SeeBlue molecular weight markers (Invitrogen). Arrow indicates biotinylated fibronectin.