ATP (100mM Stock)

Product Uses Include

• Addition to general actin buffer
• Substrate for ATPases
• Energy source for kinesin motor activity
• Energy source for kinases

Material
Adenosine triphosphate (ATP). 100 mM solution of ATP at pH 7.0 (to reduce hydrolysis) provided as a lyophilized powder. Required for G-actin stability and F-actin assembly and dynamics.

Question 1: Can the reconstituted ATP be stored at 4°, C for any length of time or does it need to be immediately snap-frozen?

Answer 1:  The ATP (Cat. # BSA04) should be reconstituted to 100 mM with 1 ml of cold 100 mM Tris, pH 7.5.  Set aside the volume that is needed for the day&rsquo, s experiment (stable for 4 h on ice) and the remaining ATP should then be aliquoted into experiment-sized amounts, snap frozen in liquid nitrogen, and stored at or below -20°, C. These stocks are stable for 6 months at -20°, C.

Question 2: How long are solutions with ATP in them stable at 4°, C if performing actin polymerization experiments?

Answer 2: Buffers containing ATP (Cat. # BSA04) will be stable for 4 h on ice.  Keep on ice until ready to use.  After 4 h discard the tube, do not re-freeze to use later.  Prepare fresh buffers (or freshly frozen aliquot) for the next experiment.

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