Actin Polymerization Buffer (10x stock)

Question 1: Once resuspended, can left-over actin polymerization buffer be stored at 4°C to maintain activity?

Answer 1: Upon reconstitution (1.8 ml of 100 mM Tris-HCl pH 7.5), the actin polymerization buffer ((Cat. #BSA02) will be at 10X strength. Set aside the volume needed for that day&rsquo, s experiments (This buffer is stable for 30 min on ice) and aliquot the remainder into 100 &mu, l volumes, snap frozen in liquid nitrogen, and stored at -70°C. These stocks are stable for 6 months at -70°C.

Question 2: After resuspension, does the actin polymerization buffer need to be used in a certain amount of time before losing activity?

Answer 2: The actin polymerization buffer ((Cat. #BSA02) will be stable for 30 min after thawing the previously frozen aliquots (or using freshly prepared). Keep on ice until ready to use. We do not recommend re-freezing aliquots.