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General Tubulin Buffer

General Tubulin Buffer

ArtNr	BST01-010
Hersteller	Cytoskeleton
Menge	1 x 100 ml
Quantity options	1 x 10 ml 1 x 100 ml 10 x 100 ml
Kategorie	Oncology By Cell Type Carcinoma Hematology Myeloma Cell Biology Cell Status Cytoskeleton Autophagy Reagents & Chemicals Biochemicals Buffers Mikrotubules By Organ Breast Cancer Testicular & Prostate Cancer
Typ	Buffer
Specific against	other
Citations	Coming soon! For the most recent publications citing this product, please contact our Technical Service department at infohoelzel.de
ECLASS 10.1	42051003
ECLASS 11.0	42051003
UNSPSC	12161700
Alias	tubulin, General tubulin buffer, General tubulin, tubulin buffer, PEM
Similar products	PEM, tubulin, General tubulin buffer, General tubulin, tubulin buffer
Versandbedingung	Raumtemperatur
Lieferbar
Shipping Temperature	AT
Storage Conditions	On Arrival: 4°C
Delivery Time	1-2 Weeks
FAQs	Question 1: How does general tubulin buffer differ from G-PEM buffer? Answer 1: Basically, these buffers are the same except that G-PEM contains 1mM GTP to support tubulin polymerization. General tubulin buffer ((Cat. #BST01) and PEM buffer typically contain 80 mM PIPES, 2 mM MgCl2, 0.5 mM EGTA pH 7.0. Both buffers should be supplemented with 1 mM GTP (hence, the &ldquo, G&rdquo, in G-PEM buffer). Question 2: Is glycerol necessary for the maintenance of biologically-active tubulin? Answer 2: Glycerol is often added to a final concentration of 5 - 10% to enhance polymerization, however, glycerol is not necessary for the maintenance of biologically active tubulin.
Weight (grams)	47
Product Uses	Resuspension and storage of tubulin
Material	General tubulin buffer (PEM).Contains 80 mM PIPES pH 6.9, 2 mM MgCl₂ and 0.5 mM EGTA. Used as a tubulin working buffer in the form of G-PEM (i.e. PEM plus 1 mM GTP (Cat.# BST06)) or a tubulin polymerization buffer in the form of glycerol containing G-PEM (PEM plus 1 mM GTP (Cat. # BST06) and e.g. 5% glycerol (see Cat. # BST05)).

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

BST01-010.pdf

BST01-010-datasheet.pdf

BST01-010-safety-datasheet.pdf