Control for Acetylation Immunoprecipitation IgG beads

On Arrival: 4°C

As part of the Signal-Seeker product line, acetyl-lysine control beads (CIG02) have been developed as highly specific negative control beads for Cytoskeleton's acetyl-lysine antibody-based affinity bead products. These control beads are far superior to beads alone, because it is a better representative of the actual IP condition. The IgG antibodies are covalently attached to minimize antibody heavy and light chain leaching, which makes interpretation of results easier. Additionally, these control beads are effective for pre-clearing lysates without risk of contamination with IgG antibody. IgG control beads (CIG02) are included in our comprehensive Signal-Seeker Acetyl-Lysine Detection Kits and utilizing these kits are recommend for first time acetyl-lysine detectiion.

Each package contains enough IgG control beads for 10 reactions.

Figure 1: COS cell either untreated or treated with TSA and nicotinamide were lysed with BlastR buffer. 1mg of protein was incubated with AAC04-beads or CIG02-beads. Samples were separated by SDS-PAGE and transferred to PVDF. Western blot using an Acetyl-lysine-HRP antibody (AAC03-HRP) was performed.