Anti-RhoA antibody (mouse MAb) + control.

AT

RhoA is one of most extensively studied member of Rho GTPase family. This family is involved in a wide range of cellular responses, including cytoskeletal reorganization, regulation of transcription, cell migration, cellular transformation and metastasis. RhoA is the predominant Rho isoform that is expressed in a large number of different cell types.

1-2 Weeks

NOTE: This product has been discontinued for individual sale

The anti-RhoA antibody (Cat. # ARH05) is a mouse monoclonal antibody that only recognizes RhoA, not , RhoB, RhoC, Rac1, Rac2, Rac3, Cdc42 or H-Ras. Other commercially available RhoA antibodies recognize one or more of these close isoforms. ARH05 is supplied as a lyophilized white powder.

Specificity and sensitivity of anti-RhoA by Western blot analysis. Recombinant small G-proteins and human platelet extract were separated by SDS-PAGE and transferred to a PVDF membrane according to the method given in this datasheet. Anti-RhoA was diluted 1 in 500 in TBST and western analysis was performed as detailed in the Western Blot Method section. Lane 1: 50 ug platelet extract, Lane 2: 10 ug platelet extract, Lane 3: 5 ug platelet extract, Lane 4: 10 ng His-RhoA, Lane 5: 5 ng His-RhoA, Lane 6: 2 ng His-RhoA, Lane 7: 100 ng His-RhoC. The recombinant and native RhoA band is visible at approximately 23 kD. No signal is visible with 100 ng of His-RhC (Lane 7). The faint bands visible above His-RhoA represent a minor species of His-RhoA doublet. Exposure time 2 minutes.