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Recombinant Agrobacterium sp.3-phosphoshikimate 1-carboxyvinyltransferase(aroA)

ArtNr CSB-YP870830ALAE-100
Hersteller Cusabio
Menge 100ug
Kategorie
Typ Proteins Recombinant
Format Liquid or Lyophilized powder
Specific against other
Host Yeast
Purity Greater than 90% as determined by SDS-PAGE.
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias 5-enolpyruvylshikimate-3-phosphate synthase,Short name:,EPSP synthase,Short name:,EPSPS
Lieferbar
Research Areas
Others
Uniprot ID
Q9R4E4
Gene Names
aroA
Organism
Agrobacterium sp. (strain CP4)
AA Sequence
MSHGASSRPATARKSSGLSGTVRIPGDKSISHRSF MFGGLASGETRITGLLEGEDVINTGKAMQAMGARI RKEGDTWIIDGVGNGGLLAPEAPLDFGNAATGCRL TMGLVGVYDFDSTFIGDASLTKRPMGRVLNPLREM GVQVKSEDGDRLPVTLRGPKTPTPITYRVPMASAQ VKSAVLLAGLNTPGITTVIEPIMTRDHTEKMLQGF GANLTVETDADGVRTIRLEGRGKLTGQVIDVPGDP SSTAFPLVAALLVPGSDVTILNVLMNPTRTGLILT LQEMGADIEVINPRLAGGEDVADLRVRSSTLKGVT VPEDRAPSMIDEYPILAVAAAFAEGATVMNGLEEL RVKESDRLSAVANGLKLNGVDCDEGETSLVVRGRP DGKGLGNASGAAVATHLDHRIAMSFLVMGLVSENP VTVDDATMIATSFPEFMDLMAGLGAKIELSDTKAA
Expression Region
1-455aa
Sequence Info
Full Length
Source
Yeast
Tag Info
N-terminal 6xHis-tagged
MW
49.6 kDa
Alternative Name(s)
5-enolpyruvylshikimate-3-phosphate synthase
Short name:
EPSP synthase
Short name:
EPSPS
Relevance
Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.
Reference
Molecular basis for the herbicide resistance of Roundup Ready crops.Funke T., Han H., Healy-Fried M.L., Fischer M., Schonbrunn E.Proc. Natl. Acad. Sci. U.S.A. 103:13010-13015(2006)
Purity
Greater than 90% as determined by SDS-PAGE.
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.
Subcellular Location
Cytoplasm
Protein Families
EPSP synthase family
Tag Information
N-terminal 6xHis-tagged

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 100ug
Lieferbar: In stock
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