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Recombinant Arabidopsis thaliana NADPH-dependent oxidoreductase 2-alkenal reductase(AER)

Recombinant Arabidopsis thaliana NADPH-dependent oxidoreductase 2-alkenal reductase(AER)

ArtNr	CSB-EP654400DOA-1
Hersteller	Cusabio
Menge	1mg
Kategorie	Peptides & Proteins Recombinant Proteins
Typ	Proteins Recombinant
Format	Liquid or Lyophilized powder
Specific against	other
Host	E.coli
Purity	Greater than 90% as determined by SDS-PAGE.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	Short name:DBR1
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Research Areas	Others
Uniprot ID	Q39172
Gene Names	P1
Organism	Arabidopsis thaliana (Mouse-ear cress)
AA Sequence	MTATNKQVILKDYVSGFPTESDFDFTTTTVELRVP EGTNSVLVKNLYLSCDPYMRIRMGKPDPSTAALAQ AYTPGQPIQGYGVSRIIESGHPDYKKGDLLWGIVA WEEYSVITPMTHAHFKIQHTDVPLSYYTGLLGMPG MTAYAGFYEVCSPKEGETVYVSAASGAVGQLVGQL AKMMGCYVVGSAGSKEKVDLLKTKFGFDDAFNYKE ESDLTAALKRCFPNGIDIYFENVGGKMLDAVLVNM NMHGRIAVCGMISQYNLENQEGVHNLSNIIYKRIR IQGFVVSDFYDKYSKFLEFVLPHIREGKITYVEDV ADGLEKAPEALVGLFHGKNVGKQVVVVARE
Expression Region	1-345aa
Sequence Info	Full Length
Source	E.coli
Tag Info	N-terminal 6xHis-SUMO-tagged
MW	54.1 kDa
Alternative Name(s)	Short name:DBR1
Relevance	Catalyzes the reduction of the 7-8 double bond of phenylpropanal substrates, such as p-coumaryl aldehyde and coniferyl aldehyde (in vitro). Has activity towards toxic substrates, such as 4-hydroxy-(2E)-nonenal (in vitro). May play a distinct role in plant antioxidant defense and is possibly involved in NAD(P)/NAD(P)H homeostasis.
Reference	Arabidopsis thaliana NADPH oxidoreductase homologs confer tolerance of yeasts toward the thiol-oxidizing drug diamide.Babiychuk E., Kushnir S., Belles-Boix E., van Montagu M., Inze D.J. Biol. Chem. 270:26224-26231(1995)
Purity	Greater than 90% as determined by SDS-PAGE.
Form	Liquid or Lyophilized powder
Buffer	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function	Involved in the detoxification of reactive carbonyls
Subcellular Location	Cytoplasm, Nucleus, nucleoplasm
Protein Families	NADP-dependent oxidoreductase L4BD family
Tissue Specificity	Expressed in leaves.
Tag Information	N-terminal 6xHis-SUMO-tagged

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