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Recombinant Bacillus thuringiensis subsp. Pesticidal crystal protein cry1Fb(cry1Fb),partial

Hersteller Cusabio
Kategorie
Typ Proteins Recombinant
Specific against other
Format Liquid or Lyophilized powder
Menge 1mg
Host E.coli
ArtNr CSB-EP528987BRS-1
Konjugat/Tag GST
eClass 6.1 34160400
eClass 9.0 42020190
Lieferbar
Alias 132KDA crystal protein;Crystaline entomocidal protoxinInsecticidal delta-endotoxin CryIF(b)
Purity Greater than 90% as determined by SDS-PAGE.
Research Areas
Others
Uniprot ID
O66377
Gene Names
cry1Fb
Organism
Bacillus thuringiensis subsp. morrisoni
AA Sequence
VKGHVDVEEQNNHRSVLVVPEWEAEVSQEVRVCPG RGYILRVTAYKEGYGEGCVTIHEVDNNTDELKFSS NCEKEQVYPGNTVACNDYNKNHGANACSSRNGGYD ESYESNSSIPADYAPVYEEEAYTDGQRGNPCEFNR GHTPLPAGYVTAELEYFPETDTVWVEIGETEGTFI VDSVELLLMEE
Expression Region
984-1169aa
Sequence Info
Partial
Source
E.coli
Tag Info
N-terminal GST-tagged
MW
47.8 kDa
Alternative Name(s)
132KDA crystal protein; Crystaline entomocidal protoxinInsecticidal delta-endotoxin CryIF(b)
Relevance
Promotes colloidosmotic lysis by binding to the midgut epithelial cells of insects.
Reference
A novel cry1Fb gene from Bacillus thuringiensis subsp. morrisoni.Song F., Zhang J., Ding Z., Chen Z., Li G., Huang D.Masuda K., Asano S.
Purity
Greater than 90% as determined by SDS-PAGE.
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
Promotes colloidosmotic lysis by binding to the midgut epithelial cells of insects.
Protein Families
Delta endotoxin family
Tag Information
N-terminal GST-tagged

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1mg
Lieferbar: In stock
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