ArtNr |
CSB-EP510777ENU-100 |
Hersteller |
Cusabio
|
Menge |
100ug |
Kategorie |
|
Typ |
Proteins Recombinant |
Format |
Liquid or Lyophilized powder |
Specific against |
other |
Host |
E.coli |
Purity |
Greater than 90% as determined by SDS-PAGE. |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Lipoate--protein ligase |
Lieferbar |
|
Research Areas |
Others |
Target / Protein |
lplA |
Biologically Active |
Not Test |
Expression System |
E.coli |
Species of origin |
Escherichia coli (strain K12 / MC4100 / BW2952) |
Uniprot ID |
C4ZT68 |
AA Sequence |
MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVL FLWRNADTVVIGRAQNPWKECNTRRMEEDNVRLAR RSSGGGAVFHDLGNTCFTFMAGKPEYDKTISTSIV LNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAY RETKDRGFHHGTLLLNADLSRLANYLNPDKKKLAA KGITSVRSRVTNLTELLPGITHEQVCEAITEAFFA HYGERVEAEIISPNKTPDLPNFAETFARQSSWEWN FGQAPAFSHLLDERFTWGGVELHFDVEKGHITRAQ VFTDSLNPAPLEALAGRLQGCLYRADMLQQECEAL LVDFPEQEKELRELSAWMAGAVR |
Tag Info |
N-terminal 6xHis-SUMO-tagged |
Expression Region |
1-338aa |
Protein Length |
Full Length |
MW |
53.9 kDa |
Alternative Name(s) |
Lipoate--protein ligase |
Relevance |
Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes. |
Reference |
Genomic sequencing reveals regulatory mutations and recombinational events in the widely used MC4100 lineage of Escherichia coli K-12. Ferenci T., Zhou Z., Betteridge T., Ren Y., Liu Y., Feng L., Reeves P.R., Wang L.J. Bacteriol. 191:4025-4029(2009) |
Purity |
Greater than 90% as determined by SDS-PAGE. |
Form |
Liquid or Lyophilized powder |
Buffer |
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
Catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes. |
Subcellular Location |
Cytoplasm |
Protein Families |
LplA family |
Tag Information |
N-terminal 6xHis-SUMO-tagged |
Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt, Sicherheitsdatenblatt und Analysezertifikat) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.
Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.