ArtNr |
CSB-EP341647ENV-100 |
Hersteller |
Cusabio
|
Menge |
100ug |
Quantity options |
1mg
10ug
100ug
20ug
200ug
50ug
500ug
|
Kategorie |
|
Typ |
Proteins Recombinant |
Format |
Liquid or Lyophilized powder |
Specific against |
other |
Host |
E.coli |
Purity |
Greater than 90% as determined by SDS-PAGE. |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Lieferbar |
|
Research Areas |
Cell Biology |
Uniprot ID |
P46850 |
Gene Names |
rtcB |
Organism |
Escherichia coli (strain K12) |
AA Sequence |
MNYELLTTENAPVKMWTKGVPVEADARQQLINTAK MPFIFKHIAVMPDVHLGKGSTIGSVIPTKGAIIPA AVGVDIGCGMNALRTALTAEDLPENLAELRQAIET AVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAG YQWLTQKYPRFLNTNNYKHLGTLGTGNHFIEICLD ESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQET LETLPSRDLAYFMEGTEYFDDYLKAVAWAQLFASL NRDAMMENVVTALQSITQKTVRQPQTLAMEEINCH HNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGS MGAKSFIVRGLGNEESFCSCSHGAGRVMSRTKAKK LFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDA VMAAQSDLVEVIYTLRQVVCVKG |
Expression Region |
1-408aa |
Sequence Info |
Full Length |
Source |
E.coli |
Tag Info |
N-terminal 6xHis-SUMO-tagged |
MW |
61.2 kDa |
Relevance |
RNA ligase that mediates the joining of broken tRNA-like stem-loop structures in case of tRNA damage. Probably participates to tRNA restriction-repair by ligating broken tRNA-like stem-loop structures with 2', 3'-cyclic phosphate and 5'-OH ends to form a splice junction with a 2'-OH, 3', 5'-phosphodiester, a step that requires GTP . Also acts as a DNA ligase in case of DNA damage by splicing 'dirty' DNA breaks, characterized by 3'-PO4 (or cyclic-PO4) and 5'-OH ends that cannot be sealed by classical DNA ligases |
Reference |
Characterization of the Escherichia coli RNA 3'-terminal phosphate cyclase and its sigma54-regulated operon. Genschik P., Drabikowski K., Filipowicz W. J. Biol. Chem. 273:25516-25526(1998) |
Purity |
Greater than 90% as determined by SDS-PAGE. |
Form |
Liquid or Lyophilized powder |
Buffer |
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
RNA ligase that mediates the joining of broken tRNA-like stem-loop structures in case of tRNA damage. Probably participates to tRNA restriction-repair by ligating broken tRNA-like stem-loop structures with 2', 3'-cyclic phosphate and 5'-OH ends to form a splice junction with a 2'-OH, 3', 5'-phosphodiester, a step that requires GTP |
Protein Families |
RtcB family |
Tag Information |
N-terminal 6xHis-SUMO-tagged |
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