ArtNr |
CSB-EP327259ENL-500 |
Hersteller |
Cusabio
|
Menge |
500ug |
Kategorie |
|
Typ |
Proteins Recombinant |
Format |
Liquid or Lyophilized powder |
Specific against |
other |
Purity |
Greater than 85% as determined by SDS-PAGE. |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Lieferbar |
|
Research areas |
Others |
Target / Protein |
traY |
Biologically active |
Not Test |
Expression system |
E.coli |
Species of origin |
Escherichia coli |
Uniprot ID |
P33789 |
AA Sequence |
MKEPKSNIRKLIDIGGLIGR |
Tag Info |
N-terminal 6xHis-SUMO-tagged |
Expression Region |
1-20aa |
Protein length |
Full Length |
MW |
18.2 kDa |
Relevance |
Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site. Relaxosome formation requires binding of IHF and TraY to the oriT region, which then facilitates binding of TraI. Also positively regulates tra gene expression |
References |
"Characterization of the oriT region of the IncFV plasmid pED208." di Laurenzio L., Frost L.S., Finlay B.B., Paranchych W. Mol. Microbiol. 5:1779-1790(1991) |
Purity |
Greater than 85% as determined by SDS-PAGE. |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
Conjugative DNA transfer (CDT) is the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site. Relaxosome formation requires binding of IHF and TraY to the oriT region, which then facilitates binding of TraI. Also positively regulates tra gene expression (By similarity). |
Subcellular Location |
Cytoplasm |
Protein Families |
TraY family |
Tag Information |
N-terminal 6xHis-SUMO-tagged |
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