ArtNr |
CSB-EP015310HU-1 |
Hersteller |
Cusabio
|
Menge |
1mg |
Kategorie |
|
Typ |
Proteins Recombinant |
Format |
Liquid or Lyophilized powder |
Specific against |
other |
Host |
E.coli |
Konjugat/Tag |
GST |
Purity |
Greater than 90% as determined by SDS-PAGE. |
ECLASS 10.1 |
32160409 |
ECLASS 11.0 |
32160409 |
UNSPSC |
12352202 |
Alias |
Cardiac myosin light chain 1 |
Lieferbar |
|
Research Areas |
Signal Transduction |
Uniprot ID |
P08590 |
Gene Names |
MYL3 |
Organism |
Homo sapiens (Human) |
AA Sequence |
MAPKKPEPKKDDAKAAPKAAPAPAPPPEPERPKEV EFDASKIKIEFTPEQIEEFKEAFMLFDRTPKCEMK ITYGQCGDVLRALGQNPTQAEVLRVLGKPRQEELN TKMMDFETFLPMLQHISKNKDTGTYEDFVEGLRVF DKEGNGTVMGAELRHVLATLGERLTEDEVEKLMAG QEDSNGCINYEAFVKHIMSS |
Expression Region |
1-195aa |
Sequence Info |
Full Length |
Source |
E.coli |
Tag Info |
N-terminal GST-tagged |
MW |
48.8 kDa |
Alternative Name(s) |
Cardiac myosin light chain 1 |
Relevance |
Regulatory light chain of myosin. Does not bind calcium. |
Reference |
Human ventricular/slow twitch myosin alkali light chain gene characterization, sequence, and chromosomal location. Fodor W.L., Darras B., Seharaseyon J., Falkenthal S., Francke U., Vanin E.F. J. Biol. Chem. 264:2143-2149(1989) |
Purity |
Greater than 90% as determined by SDS-PAGE. |
Form |
Liquid or Lyophilized powder |
Buffer |
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
Storage |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C. |
Notes |
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week. |
Function |
Regulatory light chain of myosin. Does not bind calcium. |
Involvement in disease |
Cardiomyopathy, familial hypertrophic 8 (CMH8) |
Paythway |
Cardiacmusclecontraction |
Tag Information |
N-terminal GST-tagged |
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