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Recombinant Human Diacylglycerol kinase epsilon(DGKE)

Recombinant Human Diacylglycerol kinase epsilon(DGKE)

ArtNr	CSB-CF006835HU-10
Hersteller	Cusabio
Menge	10ug
Kategorie	Peptides & Proteins Recombinant Proteins
Typ	Proteins Recombinant
Format	Liquid or Lyophilized powder
Specific against	other
Konjugat/Tag	Myc
Purity	Greater than 85% as determined by SDS-PAGE.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
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Research Topic	Signal Transduction
Uniprot ID	P52429
Gene Names	DGKE
Organism	Homo sapiens (Human)
AA Sequence	MEAERRPAPGSPSEGLFADGHLILWTLCSVLLPVF ITFWCSLQRSRRQLHRRDIFRKSKHGWRDTDLFSQ PTYCCVCAQHILQGAFCDCCGLRVDEGCLRKADKR FQCKEIMLKNDTKVLDAMPHHWIRGNVPLCSYCMV CKQQCGCQPKLCDYRCIWCQKTVHDECMKNSLKNE KCDFGEFKNLIIPPSYLTSINQMRKDKKTDYEVLA SKLGKQWTPLIILANSRSGTNMGEGLLGEFRILLN PVQVFDVTKTPPIKALQLCTLLPYYSARVLVCGGD GTVGWVLDAVDDMKIKGQEKYIPQVAVLPLGTGND LSNTLGWGTGYAGEIPVAQVLRNVMEADGIKLDRW KVQVTNKGYYNLRKPKEFTMNNYFSVGPDALMALN FHAHREKAPSLFSSRILNKAVYLFYGTKDCLVQEC KDLNKKVELELDGERVALPSLEGIIVLNIGYWGGG CRLWEGMGDETYPLARHDDGLLEVVGVYGSFHCAQ IQVKLANPFRIGQAHTVRLILKCSMMPMQVDGEPW AQGPCTVTITHKTHAMMLYFSGEQTDDDISSTSDQ EDIKATE
Expression Region	1-567aa
Sequence Info	Full Length
Source	in vitro E.coli expression system
Tag Info	N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
MW	83.9 kDa
Alternative Name(s)	Diglyceride kinase epsilon
Relevance	Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.
Reference	"Functional specialization of beta-arrestin interactions revealed by proteomic analysis." Xiao K., McClatchy D.B., Shukla A.K., Zhao Y., Chen M., Shenoy S.K., Yates J.R., Lefkowitz R.J. Proc. Natl. Acad. Sci. U.S.A. 104:12011-12016(2007)
Purity	Greater than 85% as determined by SDS-PAGE.
Storage Buffer	Tris-based buffer, 50% glycerol
Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function	Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.
Involvement in disease	Nephrotic syndrome 7 (NPHS7); Hemolytic uremic syndrome atypical 7 (AHUS7)
Subcellular Location	Membrane, Multi-pass membrane protein, Membrane, Cytoplasm
Protein Families	Eukaryotic diacylglycerol kinase family
Tissue Specificity	Expressed predominantly in testis. Expressed in endothelium, platelets and podocytes (at protein level).
Paythway	OxidativePhosphorylation
Tag Information	N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

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