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Recombinant Rat Pyruvate kinase PKM(Pkm)

Recombinant Rat Pyruvate kinase PKM(Pkm)

ArtNr	CSB-BP018072RA-10
Hersteller	Cusabio
Menge	10ug
Kategorie	Peptides & Proteins Recombinant Proteins
Typ	Proteins Recombinant
Format	Liquid or Lyophilized powder
Specific against	other
Konjugat/Tag	Myc
Purity	Greater than 85% as determined by SDS-PAGE.
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
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Research areas	Signal Transduction
Target / Protein	Pkm
Biologically active	Not Test
Expression system	Baculovirus
Species of origin	Rattus norvegicus (Rat)
Uniprot ID	P11980
AA Sequence	MPKPDSEAGTAFIQTQQLHAAMADTFLEHMCRLDI DSAPITARNTGIICTIGPASRSVEMLKEMIKSGMN VARLNFSHGTHEYHAETIKNVRAATESFASDPILY RPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATL KITLDNAYMEKCDENILWLDYKNICKVVEVGSKIY VDDGLISLQVKEKGADYLVTEVENGGSLGSKKGVN LPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFI RKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRF DEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIG RCNRAGKPVICATQMLESMIKKPRPTRAEGSDVAN AVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREA EAAVFHRLLFEELARASSQSTDPLEAMAMGSVEAS YKCLAAALIVLTESGRSAHQVARYRPRAPIIAVTR NPQTARQAHLYRGIFPVLCKDAVLDAWAEDVDLRV NLAMNVGKARGFFKKGDVVIVLTGWRPGSGFTNTM RVVPVP
Tag Info	N-terminal 10xHis-tagged and C-terminal Myc-tagged
Expression Region	1-531aa
Protein length	Full Length
MW	61.8 kDa
Alternative Name(s)	Pyruvate kinase muscle isozyme Pkm2, Pykm
Relevance	Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation
References	"The M1- and M2-type isozymes of rat pyruvate kinase are produced from the same gene by alternative RNA splicing." Noguchi T., Inoue H., Tanaka T. J. Biol. Chem. 261:13807-13812(1986)
Purity	Greater than 85% as determined by SDS-PAGE.
Storage	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function	Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation (By similarity).
Subcellular Location	Cytoplasm, Nucleus
Protein Families	Pyruvate kinase family
Tag Information	N-terminal 10xHis-tagged and C-terminal Myc-tagged

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