Human Betacellulin, BTC ELISA Kit

Human BTC ELISA Kit

Unopened Kit: Store at 2 - 8oC. Do not use past kit expiration date. Opened/Reconstituted Reagents: Unmixed TMB Solution A, Unmixed TMB Solution B, TMB Stop Solution, Wash Buffer, Sample Diluent, Antibody Diluent, HRP-avidin Diluent. The above mentioned reagents should be stored for up to 1 month at 2 - 8oC. Microplate Wells: Return unused wells to the foil pouch con-taining the desiccant pack, reseal along entire edge of zip-seal. May be stored for up to 1 month at 2 - 8oC.

1. The kit should be equilibrated to room temperature (20-23oC) before opening any vials and starting the assay. It is highly recommended that the solutions be used as soon as possible after rehydration. 2. When mixing or reconstituting protein solutions, always avoid foaming. 3. Do not mix or substitute reagents with those from other lots or sources. 4. To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent. 5. Crystals could appear in the 25X wash solution due to high salt concentration in the stock solutions. Crystals are readily dissolved at room temperature or at 37C before dilution of the buffer solutions. 6. Keep TMB Substrate protected from light. 7. The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Specificity: This assay recognizes human BTC . No signifi-cant cross-reactivity or interference was observed. Detection Rage: 0.312 ng/ml-10 ng/ml Detection Limit: The minimum detectable dose of human BTC is typically less than 0.05 ng/ml.