CARD HyperOva (Superovulation Reagent for mouse)

Shipping dry ice

KYD

CARD HyperOva^® (Superovulation Reagent for mouse) (Japanese Patent No. 5927588) is an ultra-superovulation reagent that is 3-4 times more efficient at inducing ovulation than the conventional PMSG-hCG method (80 ova per C57BL/6 mouse versus approximately 20). CARD HyperOva^® is also effective with many other strains. Even further gains in efficiency can be realized by using CARD MEDIUM^® during the IVF procedure.
Why Use CARD HyperOva^® ?

• Reduce Animal Use (contribute to the 3Rs of animal welfare: Replacement, Reduction, Refinement)
  


• Using CARD HyperOva^® can reduce the number of oocyte donor mice by a factor of 1/3-1/4.
  


• Optimize creation and preservation of genetically modified mice.
  


• More ova from fewer mice using CARD HyperOva^® facilitates in vitro fertilization and embryo transfer.
  


• More ova from fewer mice using CARD HyperOva^® enables the large-scale preparation of embryos.
  


• Get more done!
  

For information on techniques for mouse spermatozoa cryopreservation, spermatozoa thawing, in vitro fertilization and obtaining pups through embryo transfer, please refer to the CARD website: http://card.medic.kumamoto-u.ac.jp/card/english/sigen/index.html

1. Take CARD HyperOva out from the freezer, and thaw it in incubator or water bath at 37C for 10 minutes.

2. Administer the CARD HyperOva to mouse within one hour after thawing. Equalize the CARD HyperOVA by inverting the tube before aspirating it to syringe.

3. You may have some clumps floating* in the solution which you cannot aspirate by the injection needle. In such a case, avoid the clumps and aspirate only other solution.

4. Female mice (C57BL/6 strain, 4 weeks of age) are intraperitoneally administered CARD HyperOva (0.1 - 0.2mL/head; i.p).

5. At 48h after the administration of HyperOva, 7.5IU hCG(i.p.)** are administered to the mice.

6. At 17h after hCG injection, mice are sacrificed by cervical dislocation, and their oviducts are collected and transferred to a fertilization dish.