CARD FERTIUP PM 0.5 mL - CARD MEDIUM Set

IVF & Reproductive Engineering/Mouse; IVF & Reproductive Engineering/Media & Solutions

KYD

This convenient double medium set comprises CARD FERTIUP^® PM and CARD MEDIUM^® that when used together synergize to elevate fertilization rates in mouse IVF. The former is a sperm fertility-enhancing preculture medium. The latter is a novel fertilization-promoting culture medium that acts during IVF on the mouse egg zona pellucida.
CARD FERTIUP^® Preincubation Medium: PM is a preculture medium that enhances mouse sperm fertility. Used in IVF with thawed C57BL/6 mouse sperm frozen using CARD FERTIUP^® Cryoprotectant: CPA, fertilization rates of 30% or more can be expected. CARD FERTIUP^® Preincubation Medium: PM also effectively promotes IVF with fresh sperm.
CARD MEDIUM^® is a novel culture medium that acts during IVF on the mouse egg zona pellucida to promote high fertilization rates, especially when used in conjunction with CARD FERTIUP^® Preincubation Medium: PM. CARD MEDIUM^® was developed by The Center for Animal Resources and Development (CARD), Kumamoto University, Japan.
Combined use of FERTIUP^® sperm cryoprotectant and CARD FERTIUP^® Preincubation Medium: PM greatly improves the fertilization rate of frozen-thawed sperm in C57BL/6 and 129 strains, from less than 10% using conventional methods to more than 30%. Further, when coupled with our new CARD MEDIUM^® , fertility rates are boosted to over 80%. Thus, combined usage of the trio of CARD FERTIUP^® Cryoprotectant: CPA, CARD FERTIUP^® Preincubation Medium: PM and CARD MEDIUM^® provides a high and consistent rate of fertilization from frozen-thawed mouse sperm, which will allow you to efficiently recover mouse lines when needed.
For information on techniques for mouse spermatozoa cryopreservation, spermatozoa thawing, in vitro fertilization and obtaining pups through embryo transfer, please refer to the CARD website.

1. Mouse Sperm Preincubation Medium: PM 0.5ML(KYD-002-05-EX)

2. CARD MEDIUM 1 KIT (KYD-003-EX)