D-Luciferin potassium salt

115144-35-9

C11H7KN2O3S2

H2O : 12.5 mg/mL (39.26 mM; Need ultrasonic); DMSO : 2.63 mg/mL (8.26 mM; Need ultrasonic)

Others

D-Luciferin potassium salt is the substrate of luciferases that catalyze the production of light in bioluminescent insects. In Vitro: D-luciferin is the natural substrate of the enzyme luciferase (Luc), that catalyzes the production of the typical yellowgreen light of fireflies.The present review covers the synthesis of D-luciferin and derivatives or analogues that are substrates or inhibitors of the luciferase from the American firefly Photinus pyralis, the enzyme more frequently used in techniques of in vitro and optical imaging^[1]. In Vivo: Bioluminescence imaging (BLI) using the firefly luciferase (Fluc) as a reporter gene and D-luciferin as a substrate is currently the most widely employed technique. The total signal intensity is plotted against the time after D-luciferin injection to generate a time-intensity curve. In addition to the peak signal, the signals at fixed time points (5, 10, 15, and 20?min) after D-luciferin injection are determined as alternatives to the peak signal. The signal in a given time-intensity curve is normalized for the peak signal in the curve to represent the pattern of temporal changes after D-luciferin injection^[2].