(-)-Epicatechin

490-46-0

C15H14O6

DMSO : >= 34 mg/mL (117.13 mM)

Immunology/Inflammation

(-)-Epicatechin inhibits cyclooxygenase-1 (COX-1) with an IC₅₀ of 3.2 uM. (-)-Epicatechin inhibits the IL-1beta-induced expression of iNOS by blocking the nuclear localization of the p65 subunit of NF-kappaB. IC50 & Target: IC50: 3.2 uM (COX-1)^[1] In Vitro: (-)-Epicatechin exhibits >95% inhibitory activity at 70 ug/mL against cyclooxygenase-1 (COX-1) with an IC₅₀ of 3.2 uM^[1]. (-)-Epicatechin inhibits the IL-1beta-induced expression of iNOS by blocking the nuclear localization of the p65 subunit of NF-kappaB. In RINm5F cells, (-)-Epicatechin is shown to block the inhibition of insulin release after addition of IL-1beta. Additionally, (-)-Epicatechin is shown to inhibit the proliferation of Hodgkin's lymphoma cells and Jurkat T cells, which is attributed to the ability of (-)-Epicatechin to inhibit the binding of NF-kappaB to DNA in these cells. In human colorectal cancer HCT-116 cells, combining 20?uM Panaxadiol with 150, 200, or 250?uM (-)-Epicatechin results in growth inhibition of 51%, 97%, and 95%, respectively. The combination also increases the apoptosis level by 11.9%, 16.6%, and 25.8%, as examined by annexin V/PI staining^[2]. In Vivo: Animals receive 1 mg/kg of (-)-Epicatechin or water (vehicle) via oral gavage (twice daily). Exercise groups undergo 15 days of treadmill exercise. Significant increases in treadmill performance (ca.50%) and enhanced in situ muscle fatigue resistance (ca.30%) are observed with (-)-Epicatechin^[3].