5-BrdU

59-14-3

C9H11BrN2O5

DMSO : >= 41 mg/mL (133.51 mM); H2O : 33.33 mg/mL (108.53 mM; Need ultrasonic)

Cell Cycle/DNA Damage

5-BrdU is a nucleoside analog that competes with thymidine for incorporation into DNA. 5-BrdU is commonly used in the detection of proliferating cells. In Vitro: Bromodeoxyuridine induces a progressive, dose-responsive suppression of cancer cell line and cancer stem cell population expansion RG2 rat glioma cells. In H9 cells and BJ fibroblasts, bromodeoxyuridine alters the cell cycle profile^[1]. BrdU is stably integrated into the DNA, and thus can be used in assessment of cell proliferation and other cell procession^[2]. In Vivo: Bromodeoxyuridine (300 mg/kg, i.p. or 0.8 mg/mL, p.o.) significantly slows tumor progression in rat glioma RG2 tumor model^[1].