D4476

301836-43-1

C23H18N4O3

DMSO : >= 50 mg/mL (125.50 mM)

Autophagy; Stem Cell/Wnt; Cell Cycle/DNA Damage

D4476 is a potent, selective and cell-permeable inhibitor of casein kinase 1(CK1) with an IC₅₀ value of 0.3 uM in vitro. IC50 & Target: IC50: 0.3 uM (CK1)^[1] In Vitro: D4476 is a potent and rather selective inhibitor of CK1 in vitro and in cells. In H4IIE hepatoma cells, D4476 specifically inhibits the phosphorylation of endogenous forkhead box transcription factor O1a (FOXO1a) on Ser322 and Ser325 within its MPD, without affecting the phosphorylation of other sites. CK1delta assayed at 0.1 mM ATP using a phosphorylated peptide TFRPRTSpSNASTIS corresponding to residues 312–325 of FOXO1a is inhibited with an IC₅₀ value of 0.3 uM. The IC₅₀ value for CK1delta decreases progressively as the concentration of ATP is lowered, indicating that D4476 is an ATP-competitive inhibitor of CK1. CK1^[1].