PF-670462

950912-80-8

C19H22Cl2FN5

DMSO : >= 32 mg/mL (77.99 mM)

Stem Cell/Wnt; Cell Cycle/DNA Damage

PF-670462 is a potent and selective inhibitor of casein kinase (CK1epsilon and CK1delta), with IC₅₀s of 7.7 nM and 14 nM, respectively. IC50 & Target: IC50: 7.7 nM (CKIepsilon), 14 nM (CKIdelta), 150 nM (EGFR), 190 nM (SAPK2A/p38)^[1], 17 nM (Wnt/beta-catenin)^[2] In Vitro: PF-670462 is a potent and selective inhibitor of CKIepsilon and CKIdelta, with IC₅₀s of 7.7 nM and 14 nM, respectively. PF-670462 shows less than 30-fold selevtivity for EGFR and SAPK2A/p38, with IC₅₀s of 150 nM and 190 nM, respectively. PF-670462 also causes a redistribution of the GFP signal to the cytoplasm in a concentration-dependent manner, with an EC₅₀ of 290 +/- 39 nM in CKIepsilon-transfected COS7 cells^[1]. PF-670462 is a potent inhibitor of Wnt/beta-catenin signaling, with an IC₅₀ of ?17?nM. PF-670462 (1?uM) is a weak inhibitor of proliferation, and only modestly suppresses the growth of HEK293 and HT1080 cells. PF-670462 (100 nM) strongly inhibits CK1epsilon and CK1delta, consistent with its effect on Wnt/beta-catenin signaling^[2]. In Vivo: PF-670462 (50 mg/kg, s.c.) produces robust phase delays, and the activity remains persistent, with no discernible correction in the absence of exogenous zeitgebers in rats. PF-670462 (25, 50, and 100 mg/kg, s.c.) induces dose-dependent phase shift^[1]. PF-670462 (50 mg/kg; s.c.) significantly phase delays the rhythmic transcription of Bmal1, Per1, Per2 and Nr1d1 in both liver and pancreas by 4.5 +/- 1.3 h and 4.5 +/- 1.2 h, respectively, 1 day after administration. In the suprachiasmatic nucleus (SCN), the rhythm of Nr1d1 and Dbp mRNA expression is also delayed by 4.2 and 4 h, respectively^[3].