Dihydrocoumarin

119-84-6

C9H8O2

10 mM in DMSO

Epigenetics; Cell Cycle/DNA Damage

Dihydrocoumarin is a compound found in Melilotus officinalis. Dihydrocoumarin is a yeast Sir2p inhibitor. Dihydrocoumarin also inhibits human SIRT1 and SIRT2 with IC₅₀s of 208 uM and 295 uM, respectively^[1]. In Vitro: Dihydrocoumarin induces a concentration-dependent inhibition of SIRT1 (IC₅₀ of 208 uM) in an in vitro enzymatic assay. A decrease in SIRT1 deacetylase activity is observed even at micromolar doses (85+/-5.8 and 73+/-13.7% activity at 1.6 uM and 8 uM, respectively). The microtubule SIRT2 deacetylase is also inhibited with a similar dose dependency (IC₅₀ of 295 uM)^[1].
Dihydrocoumarin (1-5 mM) increases cytotoxicity in the TK6 cell line in a dose-dependent manner following a 24-h exposure. Dihydrocoumarin (1-5 mM) increases apoptosis in a dose-dependent manner in the TK6 cell line at the 6-h time point. A 5-mM dose of Dihydrocoumarin increases apoptosis at the 6-h time point in the TK6 cell line^[1].
Dihydrocoumarin (1-5 mM) increases p53 lysine 373 and 382 acetylation in a dose-dependent manner in the TK6 cell line following a 24-h exposure period^[1].