ArtNr |
CLS-604328 |
Hersteller |
CLS Cell Lines Service
|
Menge |
1 cryovial |
Kategorie |
|
Typ |
Cell line |
Specific against |
other |
Dry ice |
Yes
|
ECLASS 10.1 |
42040401 |
ECLASS 11.0 |
42040401 |
UNSPSC |
41106509 |
Alias |
SF9, sf9, SF-9, Sf-9, sf-9, Sf 9, Spodoptera frugiperda clone 9, Sf clone 9, IPLB-Sf-9AE, IPLB-SF-9AE, IPLB-SF-9, IPLB-Sf-9, IPLB-Sf9 |
Lieferbar |
|
Manufacturers Category |
Insect cell line |
Organism |
Fall armyworm |
Tissue |
Ovary |
Applications |
Transfection host |
Age |
Pupal stage |
Gender |
Female |
Morphology |
Round, attached, epitheloid |
Growth Properties |
Monolayer, adherent |
Biosafety Level |
1 |
Virus Susceptibility |
Baculoviruses, Autographa californica (MNPV), St. Louis encephalitis (SLE) |
Culture Medium |
Medium for Sf9 cells is available from different suppliers. For example Spodopan (PAN Biotech) is recommended. |
Medium Supplements |
Addition of 2% FBS enhances proliferation. Use cold medium for medium exchanges or seeding of cells. |
Subculturing |
Detachment of cells via a cell scraper is recommended. Collect the medium with detached cells after scraping in a 15ml centrifuge tube. Add about 5ml of medium to the flask and rinse the flask several times to collect any remaining cells and combine them with the rest of the cells in the tube. Centrifuge for 3 min at 300xg, remove the supernatant, resuspend the cells in fresh, cold medium and dispense into new flasks. |
Split Ratio |
For the first two subcultivations a ratio of 1:3 to 1:5 is recommended. In further subcultivations cells can be split at a ratio of 1:10 to 1:20 |
Seeding Density |
1 x 10^4 cells/cm^2. Incubate between 26 to 30?C in a nontohumidified, ambient airtoregulated incubator. Use cell culture flasks with filter caps or loosen caps to allow for oxygen exchange. |
Fluid Renewal |
2 to 3 times per week |
Freeze Medium |
|
Amelogenin |
X, X |
NOTE |
Deutsch:Universitäre Kunden: Für den Erwerb ist ein Material Transfer Agreement oder eine Limited Use Label License auszufüllen. Kommerzielle Kunden: Für den Erwerb ist ein Material Transfer Agreement oder ein Master Supply Agreement auszufüllen.Nach eingegangener Bestellung werden ihnen alle relevanten Dokumente zugeschickt.English:University Customers: A Material Transfer Agreement or Limited Use Label License must be completed for purchase.Commercial Customers: A Material Transfer Agreement or Master Supply Agreement must be completed for purchase.After the order is received, all relevant documents will be sent to you. |
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